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001-es BibID:	BIBFORM027917
Első szerző:	Vissi Emese (biokémikus, biológus)
Cím:	Protein phosphatase 1 catalytic subunit isoforms from alfalfa : biochemical characterization and cDNA cloning / Emese Vissi, Éva Csordás Tóth, Izabella Kovács, Zoltán Magyar, Gábor V. Horváth, Péter Bagossi, Pál Gergely, Dénes Dudits, Viktor Dombrádi
Dátum:	1998
Megjegyzések:	The catalytic subunit of protein phosphatase 1 (PP1c) was purified from an alfalfa (Medicago sativa) microcallus cell culture. The preparation was inhibited by rabbit muscle inhibitor-2 and okadaic acid and had a molecular mass of 35 kDa. Five distinct cDNAs termed MsPP1alpha, -beta, -gamma, -delta, and -epsilon were cloned from a M. sativa somatic embryo library. MsPP1alpha was identical to a cDNA reported earlier [A. Páy, M. Pirck, L. Bögre, H. Hirt, and E. Heberle-Bors Mol. Gen. Genet. 244, 176-182, 1994], while the others represented novel isoforms encoded by separate genes. The predicted amino acid sequences of MsPP1alpha, -beta, -gamma, -delta, and -epsilon were highly similar to each other and to other known PP1c sequences. The GST-MsPP1ss fusion protein expressed in Escherichia coli was catalytically active and was inhibited by inhibitor-2 and okadaic acid. Affinity-purified polyclonal MsPP1antipeptide antibody detected a protein of 36 kDa in crude cell extracts. These results proved that the cDNA clone encoded an active PP1c which was very similar to the purified enzyme. The mRNA and protein concentrations of PP1c as well as the specific activity of protein phosphatase 1 did not change during the cell cycle in a synchronized alfalfa cell culture. On the other hand, the isoforms exhibited different steady-state mRNA levels in different plant organs suggesting tissue-specific functions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban protein phosphatase1 Medicago sativa protein purification cDNA sequencing cell cycle gene expression egyetemen (Magyarországon) készült közlemény
Megjelenés:	Archives of Biochemistry and Biophysics. - 360 : 2 (1998), p. 206-214. -
További szerzők:	Csordás Tóth Éva Kovács Izabella (Szeged) Magyar Zoltán Horváth Gábor V. Bagossi Péter (1966-2011) (biokémikus, vegyész) Dudits Dénes Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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