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001-es BibID:	BIBFORM030324
035-os BibID:	(PMID)22128034
Első szerző:	Damjanovich László (általános sebész)
Cím:	Crohn's disease alters MHC-rafts in CD4+ T-cells / László Damjanovich, Julianna Volkó, Attila Forgács, Werner Hohenberger, László Bene
Dátum:	2012
ISSN:	1552-4922 1552-4930
Megjegyzések:	Clusters of MHCI, ICAM-1, CD44, CD59, IL-2R, and IL-15R molecules have been studied on the surface of CD4(+) T-cells from peripheral blood and lymph nodes of patients in Crohn's disease and healthy individuals as controls by using a dual-laser flow cytometric fluorescence resonance energy transfer (FRET) technique and fluorescently stained Fabs. When cells from patients in Crohn's disease are compared to those of controls, the surface expression level for the MHCI reduced by ?45%, for CD44 enhanced by ?100%, and for IL-2R?, IL-15R?, and common ?(c) enhanced by ?50%, ?70%, and ?130%, respectively. Efficiencies of FRET monitoring homoassociation for the MHCI and CD44 reduced, that for IL-2R? enhanced. While efficiencies of FRET monitoring the association of ?(c) and ICAM-1 with the MHCI reduced, those monitoring association of IL-2/15R?, CD44, and CD59 with MHCI enhanced. Efficiencies of FRET measured between the MHCI and IL-2R?, IL-15R? differently enhanced to the advantage of IL-15R?, the one measured between ?(c) and IL-2R? reduced, suggesting modulations in the strength of interaction of MHCI with IL-2R, IL-15R, and ?(c) . The increases in density of surface bound cTx and in the associations of the receptors with the G(M1) -ganglioside lipid molecules suggest stronger lipid raft interactions of the receptors. The observed alterations of MHC-rafts in Crohn's disease-summarized in models of receptor patterns of diseased and control cells-may have functional consequences regarding signaling by the raft components.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Molekuláris Medicina MHC class I ICAM-1 IL-2R/15R CD44 CD59 Crohn's disease draining lymph node CD4+ T-cells lipid raft fluorescence resonance energy transfer
Megjelenés:	Cytometry. Part A. - 81A : 2 (2012), p. 149-164. -
További szerzők:	Volkó Julianna (1983-) (biotechnológus) Forgács Attila (1985-) (fizikus) Hohenberger, Werner Bene László (1963-) (biofizikus)
Pályázati támogatás:	K77600 OTKA TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Sejt biofizika
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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