Találati lista | Tudóstér

001-es BibID:	BIBFORM030855
Első szerző:	Bubán Tamás (belgyógyász, gasztroenterológus)
Cím:	Detection of mutations in the cDNA of the proliferation marker Ki-67 protein in four tumor cell lines / Tamás Bubán, Mirko Schmidt, Rainer Broll, Péter Antal-Szalmás, Michael Duchrow
Dátum:	2004
ISSN:	0165-4608
Megjegyzések:	The Ki-67 protein has an essential role in cell proliferation. It is present in all dividing cells ofnormal and tumor tissues, but absent in resting cells. At present, no data are available about anyalterations in the gene of this protein that could contribute to its altered structure and function,resulting in tumor development. We therefore searched for mutations in the Ki-67 gene (MKI67).cDNAs from four tumor cell lines derived from carcinoma of the cervix (HeLa), colon (CXF94,SW480), and lung (A549) were prepared. Defined parts of the cDNA were amplified by specificprimers, cloned into pCRII-Blunt-TOPO vector, and replicated in Escherichia coli. The sequenceof the amplified products were determined by automated fluorescence sequencing. Eight differentmutations were characterized in the four cell lines tested. One is a deletion of a single base at position1496 causing a truncated protein, the second is a A433T exchange is a silent mutation, and theremaining six mutations result in an amino acid change thatmight alter the conformation of the protein.Our results show that several mutations exist within the Ki-67 protein's cDNA in four tumor celllines. These mutations might provide a genetic basis for tumor development.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Molekuláris Medicina Ki-67 tumor cell lines mutation külföldön készült közlemény
Megjelenés:	Cancer Genetics And Cytogenetics 149 : 1 (2004), p. 81-84. -
További szerzők:	Schmidt, Mirko Broll, Rainer Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Duchrow, Michael
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Sejtbiológia DAAD (A/00/13866) Egyéb Sejtbiológia
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: