Találati lista | Tudóstér

001-es BibID:	BIBFORM031435
035-os BibID:	(Scopus)84856037778 (WoS)A1996VC67200013
Első szerző:	Fernández, Imma
Cím:	Conformation and self-association of a hybrid peptide of cecropin A and melittin with improved antibiotic activity / Imma Fernandez, Josep Ubach, Monika Fuxreiter, José Manuel Andreu, David Andreu, Miquel Pons
Dátum:	1996
ISSN:	0947-6539
Megjegyzések:	A 15-residue hybrid peptide containing residues 1-7 from cecropin A and residues 2-9 from melittin, (1-7)M(2-9), is a potent antibiotic with broader activity than cecropin A, bur without the cytotoxic character of melittin. The conformational behaviour of CA(1-7)M(2-9) including the formation of multimeric species in solution has been investigated by circular dichroism, ultracentrifugation, electrospray mass spectrometry, NMR and energy calculations. Addition of hexafluoroisopropanol or liposomes causes the appearance of a CD spectrum characteristic of a helical structure that changes with pH, buffer and peptide concentration. The concentration dependence is atypical, as the ellipticity at 222 nm decreases with peptide concentration and is not correlated with a corresponding decrease in helix content as measured from the NMR spectra. The presence of aggregated structures is demonstrated by ultracentrifugation and ES-MS experiments, which also provide an indication of the stoichiometry. Long-range NOEs suggest a model of aggregation with neighbouring molecules packed antiparallel. Aggregation causes very slow proton-deuterium exchange in some amide protons in the C-terminal region and provides a method for estimating a very large association constant (ca. 10(6) M(-1)) as well as the stoichiometry of the aggregates. The tendency to aggregate seems to be an inherited feature from melittin and may enhance the antibiotic activity either by facilitating the incorporation of the peptide into the membrane in large quantities or by promoting the disruption of the membrane.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Chemistry : a Eueopean Journal. - 2 : 7 (1996), p. 838-846. -
További szerzők:	Ubach, Josep Fuxreiter Mónika (1969-) (kutató vegyész) Andreu, José Manuel Andreu, David Pons, Miquel
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
Borító:
Saját polcon: