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001-es BibID:	BIBFORM032364
035-os BibID:	PMID:2173695
Első szerző:	Csépány Tünde (neurológus, pszichiáter)
Cím:	Sequence specificity of mRNA N6-adenosine methyltransferase / Tunde Csepany, Alison Lin, Carl J. Baldick Jr., Karen Beemon
Dátum:	1990
Megjegyzések:	The sequence specificity of chicken mRNA N6-adenosine methyltransferase has been investigated in vivo. Localization of six new N6-methyladenosine sites on Rous sarcoma virus (RSV) virion RNA has confirmed our extended consensus sequence for methylation: RGACU, where R is usually a G (7/12). We have also observed A (2/12) and U (3/12) at the -2 position (relative to m6A at +1) but never a C. At the +3 position, the U was observed 10/12 times; an A and a C were observed once each in weakly methylated sequences. The extent of methylation varied between the different sites up to a maximum of about 90%. To test the significance of this consensus sequence, it was altered by site-specific mutagenesis, and methylation was assayed after transfection of mutated RSV DNA into chicken embryo fibroblasts. We found that changing the G at -1 or the U at +3 to any other residue inhibited methylation. However, inhibition of methylation at all four of the major sites in the RSV src gene did not detectably alter the steady-state levels of the three viral RNA species or viral infectivity. Additional mutants that inactivated the src protein kinase activity produced less virus and exhibited relatively less src mRNA in infected cells.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	The Journal of biological chemistry. - 265 : 33 (1990), p. 20117-20122. -
További szerzők:	Lin, Alison Baldick, Carl J. Jr. Beemon, Karen
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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