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001-es BibID:	BIBFORM033451
Első szerző:	Daoud, Rosette
Cím:	Ischemia induces a translocation of the splicing factor tra2-beta 1 and changes alternative splicing patterns in the brain / Daoud R., Mies G., Smialowska A., Oláh L., Hossmann K. A., Stamm S.
Dátum:	2002
Megjegyzések:	Alternative splice-site selection is regulated by the relative concentration of individual members of the serine-arginine family of proteins and heterogeneous nuclear ribonucleoproteins. Most of these proteins accumulate predominantly in the nucleus, and a subset of them shuttles continuously between nucleus and cytosol. We demonstrate that in primary neuronal cultures, a rise in intracellular calcium concentration induced by thapsigargin leads to a translocation of the splicing regulatory protein tra2-beta1 and a consequent change in splice-site selection. To investigate this phenomenon under physiological conditions, we used an ischemia model. Ischemia induced in the brain causes a cytoplasmic accumulation and hyperphosphorylation of tra2-beta1. In addition, several of the proteins binding to tra2-beta1, such as src associated in mitosis 68 and serine/arginine-rich proteins, accumulate in the cytosol. Concomitant with this subcellular relocalization, we observed a change in alternative splice-site usage of the ICH-1 gene. The increased usage of its alternative exons is in agreement with previous studies demonstrating its repression by a high concentration of proteins with serine/arginine-rich domains. Our findings suggest that a change in the calcium concentration associated with ischemia is part of a signaling event, which changes pre-mRNA splicing pathways by causing relocalization of proteins that regulate splice-site selection.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	The Journal of Neuroscience. - 22 : 14 (2002), p. 5889-5899. -
További szerzők:	Mies, Günter Smialowska, Agata Oláh László (1967-) (neurológus) Hossmann, Konstantin-Alexander Stamm, Stefan
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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