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001-es BibID:	BIBFORM033987
Első szerző:	Knüver, Jana
Cím:	Thyrotropin-releasing hormone controls mitochondrial biology in human epidermis / Knuever J., Poeggeler B., Gáspár E., Klinger M., Hellwig-Burgel T., Hardenbicker C., Tóth B. I., Bíró T., Paus R.
Dátum:	2012
ISSN:	0021-972X
Megjegyzések:	Context: Mitochondrial capacity and metabolic potential are under the control of hormones, such as thyroid hormones. The most proximal regulator of the hypothalamic-pituitary-thyroid (HPT) axis, TRH, is the key hypothalamic integrator of energy metabolism via its impact on thyroid hormone secretion. Objective: Here, we asked whether TRH directly modulates mitochondrial functions in normal, TRH-receptor-positive human epidermis. Methods: Organ-cultured human skin was treated with TRH (5-100 ng/ml) for 12-48 h. Results: TRH significantly increased epidermal immunoreactivity for the mitochondria-selective subunit I of respiratory chain complex IV (MTCO1). This resulted from an increased MTCO1 transcription and protein synthesis and a stimulation of mitochondrial biogenesis as demonstrated by transmission electron microscopy and TRH-enhanced mitochondrial DNA synthesis. TRH also significantly stimulated the transcription of several other mitochondrial key genes (TFAM, HSP60, and BMAL1), including the master regulator of mitochondrial biogenesis (PGC-1 alpha). TRH significantly enhanced mitochondrial complex I and IV enzyme activity and enhanced the oxygen consumption of human skin samples, which shows that the stimulated mitochondria are fully vital because the main source for cellular oxygen consumption is mitochondrial endoxidation. Conclusions: These findings identify TRH as a potent, novel neuroendocrine stimulator of mitochondrial activity and biogenesis in human epidermal keratinocytes in situ. Thus, human epidermis offers an excellent model for dissecting neuroendocrine controls of human mitochondrial biology under physiologically relevant conditions and for exploring corresponding clinical applications. (J Clin Endocrinol Metab 97: 978-986, 2012)
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Clinical Endocrinology & Metabolism 97 : 3 (2012), p. 978-986. -
További szerzők:	Poeggeler, Burkhard Gáspár E. Klinger, Matthias Hellwig-Burgel, T. Hardenbicker, Celine Tóth István Balázs (1978-) (élettanász) Bíró Tamás (1968-) (élettanász) Paus, Ralf
Internet cím:	DOI elektronikus változat Intézményi repozitóriumban (DEA) tárolt változat
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