Találati lista | Tudóstér

001-es BibID:	BIBFORM034556
Első szerző:	Bodor Csaba
Cím:	Angiotensin II increases the permeability and PV-1 expression of endothelial cells / Bodor, C., Nagy, J. P., Vegh, B., Nemeth, A., Jenei, A., MirzaHosseini, S., Sebe, A., Rosivall, L.
Dátum:	2012
Megjegyzések:	Angiotensin II (ANG II), the major effector molecule of the renin-angiotensin system (RAS), is a powerful vasoactive mediator associated with hypertension and renal failure. In this study the permeability changes and its morphological attributes in endothelial cells of human umbilical vein (HUVECs) were studied considering the potential regulatory role of ANG II. The effects of ANG II were compared with those of vascular endothelial growth factor (VEGF). Permeability was determined by 40 kDa FITC-Dextran and electrical impedance measurements. Plasmalemmal vesicle-1 (PV-1) mRNA levels were measured by PCR. Endothelial cell surface was studied by atomic force microscopy (AFM), and caveolae were visualized by transmission electron microscopy (TEM) in HUVEC monolayers. ANG II (10(-7) M), similarly to VEGF (100 ng/ml), increased the endothelial permeability parallel with an increase in the number of cell surface openings and caveolae. AT1 and VEGF-R2 receptor blockers (candesartan and ZM-323881, respectively) blunted these effects. ANG II and VEGF increased the expression of PV-1, which could be blocked by candesartan or ZM-323881 pretreatments and by the p38 mitogem-activated protein (MAP) kinase inhibitor SB-203580. Additionally, SB-203580 blocked the increase in endothelial permeability and the number of surface openings and caveolae. In conclusion, we have demonstrated that ANG II plays a role in regulation of permeability and formation of cell surface openings through AT1 receptor and PV-1 protein synthesis in a p38 MAP kinase-dependent manner in endothelial cells. The surface openings that increase in parallel with permeability may represent transcellular channels, caveolae, or both. These morphological and permeability changes may be involved in (patho-) physiological effects of ANG II
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Angiotensin II article biosynthesis Capillary Permeability Carrier Proteins egyetemen (Magyarországon) készült közlemény Caveolae cell surface Cells Cells,Cultured Comparative Study cytology Endothelial Cells Endothelium,Vascular Gene Expression Regulation,Developmental genetics Human Human Umbilical Vein Endothelial Cells Humans Hungary Membrane Proteins metabolism Microscopy Permeability physiology protein synthesis Protein Transport Proteins Receptor,Angiotensin,Type 1 Research Research Support Support
Megjelenés:	American Journal of Physiology. Cell physiology. - 302 : 1 (2012), p. C267-C276. -
További szerzők:	Nagy János Péter Végh Borbála Németh Adrienn Jenei Attila (1966-) (biofizikus) MirzaHosseini, Shahrokh Sebe Attila Rosivall László (1949-)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: