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001-es BibID:	BIBFORM036863
035-os BibID:	PMID:11843824
Első szerző:	Kappelmayer János (laboratóriumi szakorvos)
Cím:	Identification of P-selectin glycoprotein ligand-1 as a useful marker in acute myeloid leukaemias / János Kappelmayer, Attila Kiss, Éva Karászi, Anikó Veszprémi, János Jakó, Csongor Kiss
Dátum:	2001
Megjegyzések:	Immunophenotyping is considered to be less valuable in the diagnosis of acute myeloid leukaemias (AML) compared with acute lymphoid leukaemias. Here, we present data on the use of quantitative flow cytometry (QFC) of P-selectin glycoprotein ligand 1 (PSGL-1, CD162) and three-colour immunophenotyping including CD162 staining in the identification of myeloid precursors in AML. Analysis of normal peripheral blood (n = 20) and normal bone marrow (n = 5) samples and on 20 samples from de novo M1, M2, M4 and M5 AML patients demonstrated that PSGL-1 is differentially expressed on various mature and immature leucocyte subsets. It was found by QFC that neutrophils expressed 26500 +/- 4500 and monocytes 47200 +/- 9900 copies of PSGL-1 on their surface, whereas AML blasts from M1 and M2 AML patients expressed significantly less PSGL-1 (12 000 +/- 5300) than mature neutrophils (P < 0.001). In M4 and M5 leukaemias, however, the amount of PSGL-1 on monocytic precursors is displayed in a fairly broad range that is not significantly different from that of mature monocytes (P = 0.084). Using three-colour immunophenotyping PSGL-1-dim staining was co-expressed with CD7 and C34 positivity and PSGL-1 staining intensity on immature myeloid cells paralleled with CD45 expression. This would imply a differential expression of PSGL-1 during myeloid haematopoietic development and suggests that quantification of surface PSGL-1 may aid in differentiating myeloblasts from monoblasts by immunophenotyping in different AML subsets.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban P-selectin glycoprotein ligand-1 egyetemen (Magyarországon) készült közlemény
Megjelenés:	British Journal of Haematology. - 115 : 4 (2001), p. 903-909. -
További szerzők:	Kiss Attila (1942-) (belgyógyász, haematológus) Karászi Éva Veszprémi Anikó Jakó János Kiss Csongor (1956-) (hematológus, onkológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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