Találati lista | Tudóstér

001-es BibID:	BIBFORM037603
Első szerző:	Varga Zoltán (biofizikus, szakfordító)
Cím:	Potassium channel expression in human CD4(+) regulatory and naive T cells from healthy subjects and multiple sclerosis patients / Zoltan Varga, Tunde Csepany, Ferenc Papp, Akos Fabian, Peter Gogolak, Agnes Toth, Gyorgy Panyi
Dátum:	2009
ISSN:	0165-2478
Megjegyzések:	The membrane potential of human T cells is regulated by two potassium channels: the voltage-gatedKV1.3 and the Ca2+-activated KCa3.1. These two channels are essential for efficient antigenic activation andproliferation of T cells and are expressedat different levels in naïve, centralmemory and effectormemory Tcells. This provides the opportunity to inhibit the proliferation of the targeted subtype by channel-specificblocking compounds. Regulatory T cells (Tregs) also represent a unique subtype of T cells that performhighly specialized tasks in controlling immune responses, which raises the possibility that they too havea distinctive channel expression pattern. Using whole-cell patch-clamp we tested this hypothesis anddetermined the ion channel expression of CD4+CD25hiCD127lo regulatory and CD4+CD25loCD127hi naïveT cells from the peripheral blood of healthy volunteers and multiple sclerosis (MS) patients sorted by flowcytometry. We have found that naïve and Treg cells from healthy controls expressed equal numbers ofKV1.3 channels, while Tregs had a greater membrane surface as assessed by capacitance measurements,and consequentially lower channel density than naïve cells, indicating an "incomplete activation state"of Tregs. In contrast, Tregs from MS patients had fewer KV1.3 channels than naïve cells and there wasno difference in the membrane capacitance or channel density between the two subtypes of cells. Theexpression level of KCa3.1 channels was similar in all cell subsets. The observed differences in KV1.3channel expression density may contribute to the varying responses upon antigenic stimulation by thesecell types in health and disease.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Regulatory T cell Potassium channel Multiple sclerosis Cell capacitance Channel density egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters 124 : 2 (2009), p. 95-101. -
További szerzők:	Csépány Tünde (1956-) (neurológus, pszichiáter) Papp Ferenc (1979-) (biofizikus) Fábián Ákos István (1982-) (aneszteziológus) Gogolák Péter (1968-) (biológus, immunológus) Tóth Ágnes (1983-) (biofizikus) Panyi György (1966-) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
Borító:
Saját polcon: