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001-es BibID:	BIBFORM038832
Első szerző:	Emri Gabriella (bőrgyógyász, allergológus, onkológus)
Cím:	Low doses of UVB or UVA induce chromosomal aberrations in cultured hu man skin cells / Emri, G., Wenczl, E., van Erp, P., Jans, J., Roza, L., Horkay, I., Schothorst, A. A.
Dátum:	2000
Megjegyzések:	Chromosomal defects are frequently present in malignant and premalignant skin disorders; however, it is not known whether ultraviolet radiation from sunlight plays a role in their induction. To obtain information on the ability of ultraviolet A and ultraviolet B to induce chromosomal aberrations, cultured melanocytes and fibroblasts were exposed to physiologic doses of ultraviolet A or ultraviolet B and, for comparison, to gamma rays. As a measure of chromosomal aberrations, the formation of micronuclei was determined. To obtain sufficient statistical data on induced micronuclei and cell kinetics, a flow cytometry method has been modified and applied. The flow cytometry method analysis is based on staining the DNA with ethidium bromide and the cell membranes with 1,6-diphenyl-1,3,5,-hexatriene. We observed dose-dependent micronuclei formation after gamma or ultraviolet B irradiation in both cell types and also for ultraviolet A in fibroblasts. The yield of micronuclei induced in fibroblasts by ultraviolet A was only a factor 15 smaller than that induced by ultraviolet B (313 nm). The results indicate that 10 kJ per m2 (equivalent to 1 minimal erythema dose) of ultraviolet B and 150 kJ per m2 of ultraviolet A (0.2 minimal erythema dose) can induce 1% of micronuclei in fibroblasts, equivalent to the induction due to 0.6 Gy of gamma radiation. In conclusion, physiologic doses of sunlight can induce chromosomal aberrations at a level comparable with that observed after exposure to approximately 1 Gy of ionizing radiation. Therefore, sunlight can be considered a potential inducer of chromosomal aberrations in skin cells, which may contribute to skin carcinogenesis.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Investigative Dermatology. - 115 : 3 (2000), p. 435-440. -
További szerzők:	Wenczl Enikő Erp, Piet van Jans, Judith Roza, Len Horkay Irén (1938-) (bőrgyógyász, labor szakorvos, kozmetológus) Schothorst, Albert A.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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