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001-es BibID:	BIBFORM040018
Első szerző:	Nagy Henrietta
Cím:	P-Glycoprotein conformational changes detected by antibody competition / Nagy Henrietta, Goda Katalin, Arceci Robert, Cianfriglia Maurizio, Mechetner Eugene, Szabó Gábor jr.
Dátum:	2001
ISSN:	0014-2956
Megjegyzések:	Conformational changes accompanying P-glycoprotein (Pgp) mediated drug transport are reflected by changes in the avidity of certain monoclonal antibodies (mAbs). More of the UIC2 mAb binds to Pgp-expressing cells in the presence of substrates or modulators [Mechetner, E.B., Schott, B., Morse, S.B., Stein, W., Druley, T., Dvis, K.A., Tsuruo, T. & Roninson, I.B. (1997) Proc. Natl Acad. Sci. USA 94, 12908-12913], while the binding of other mAbs (e.g. MM12.10, MRK16, 4E3) is not conformation sensitive. Pre-staining of Pgp+ cells with UIC2 decreased the subsequent binding of MM12.10 mAb by about 30-40%, suggesting that there are Pgp molecules available for both UIC2 and MM12.10, and others accessible only for MM12.10. In the presence of certain substrates/modulators such as vinblastin, cyclosporin A or valinomycin, the MM12.10 reactivity was completely abolished by preincubation with UIC2. However, verapamil, Tween-80 and nifedipine did not influence the ratio of bound mAbs significantly. This is the first assay to our knowledge, sharply distinguishing two classes of modulators. The conformational changes accompanying the mAb competition phenomenon appear to be closely related, though not identical to those accompanying the UIC2-shift, as suggested by the simultaneous assessment of the two phenomena.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban 3T3 Cells Animal Antibiotics Antibiotics,Peptide Antibodies Antibodies,Monoclonal Antineoplastic Agents Antineoplastic Agents,Phytogenic Binding,Competitive Biophysics Calcium Calcium Channel Blockers Cells chemistry Cyclosporine Drug Resistance,Neoplasm Enzyme Inhibitors Flow Cytometry Human Hungary metabolism Mice Nifedipine P-Glycoprotein pharmacology Polysorbates Protein Binding Protein Conformation Substrate Specificity Support,Non-U.S.Gov't Tumor Cells,Cultured Valinomycin Verapamil Vinblastine
Megjelenés:	European Journal Of Biochemistry. - 268 : 8 (2001), p. 2416-2420. -
További szerzők:	Goda Katalin (1969-) (biofizikus) Arceci, Robert Cianfriglia, Maurizio Mechetner, Eugene Szabó Gábor (1980-) (orvos) jr
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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