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001-es BibID:	BIBFORM040247
Első szerző:	Németh József (vegyész, analitikus)
Cím:	Effect of pituitary adenylate cyclase activating polypeptide-38 on sensory neuropeptide release and neurogenic inflammation in rats and mice / J. Németh, D. Reglödi, G. Pozsgai, Á. Szabó, K. Elekes, E. Pintér, J. Szolcsányi, Z. Helyes
Dátum:	2006
ISSN:	0306-4522
Megjegyzések:	Substance P (SP) and calcitonin gene-relatedpeptide (CGRP), released from capsaicin-sensitive sensorynerves induce local neurogenic inflammation, while somatostatinexerts systemic anti-inflammatory actions. The aimof the present study was to investigate the release of pituitaryadenylate cyclase activating polypeptide-38 (PACAP-38) andits effects on sensory neuropeptide release in vitro and acuteneurogenic ear swelling in vivo.Capsaicin (10-6 M) or electrical field stimulation (EFS; 40V, 0.1 ms, 10 Hz, 120 s; 1200 impulses)-induced release ofPACAP-38, SP, CGRP and somatostatin from isolated rattracheae was measured with radioimmunoassay. Mustardoil?induced neurogenic inflammation in the mouse ear wasdetermined with a micrometer and in the rat hind paw skin bythe Evans Blue leakage technique.Capsaicin and EFS evoked 27% and more than twofoldelevation of PACAP-38 release respectively, compared withthe prestimulated basal values from isolated trachea preparation.Exogenously administered PACAP-38 (20?2000 nM)diminished both capsaicin- and EFS-evoked sensory neuropeptiderelease in a concentration-dependent manner. Themaximal inhibitory effects of PACAP on capsaicin-inducedsubstance P, CGRP and somatostatin release amounted to75.4%, 73.3% and 90.0%, while EFS-evoked release of thesepeptides was 80.03%, 87.7% and 67.7%. In case of capsaicinstimulation the EC50 values for substance P, CGRP and somatostatinwere 82.9 nM, 60.1 nM and 66.9 nM, respectively.When EFS was performed, these corresponding EC50 datawere 92.1 nM, 67.8 nM and 20.9 nM. PACAP-38 (10, 100 and1000 g/kg i.p. in 200 l volume) inhibited neurogenic earswelling in the mouse. Furthermore, 100 g/kg i.p. PACAPalso significantly diminished mustard oil?evoked plasmaprotein extravasation in the rat skin.These results suggest that PACAP-38 is released from thestimulated peripheral terminals of capsaicin-sensitive afferentsand it is able to inhibit the outflow of sensory neuropeptides.Based on this mechanism of action PACAP is also ableto effectively diminish/abolish neurogenic inflammatory responsein vivo after systemic administration.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Neuroscience. - 143 : 1 (2006), p. 223-230. -
További szerzők:	Reglődi Dóra (Idegtudományok) Pozsgai Gábor Szabó Á. Elekes K. Pintér E. Szolcsányi János (Pécs) Helyes Zsuzsanna
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