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001-es BibID:	BIBFORM043404
035-os BibID:	PMID:11684129
Első szerző:	Katona Éva (klinikai biokémikus)
Cím:	Enzyme-linked immunosorbent assay for the determination of blood coagulation factor XIII A-subunit in plasma and in cell lysates / Éva Katona, Éva Ajzner, Katalin Tóth, Levente Kárpáti, László Muszbek
Dátum:	2001
ISSN:	0022-1759
Megjegyzések:	A new one-step ELISA was developed for the determination of the concentration of blood coagulation factor XIII subunitA ZFXIII-A. in plasma and in cell lysates. Monoclonal antibodies directed against different epitopes on FXIII-A were usedfor the assay. The capture antibody was biotinylated on its carbohydrate moiety and the detection antibody was labelled withhorseradish peroxidase. The antigen?antibody reaction was carried out in the well of a streptavidin-coated microplate.Complex formation with FXIII subunit B ZFXIII-B. and association to fibrinogen did not influence the accessibility of theantibodies to FXIII-A. The method could be performed within 2 h and demonstrated good reproducibility, recovery andsensitivity. Plasma samples could be assayed after storage at y20 8C for at least 6 months. However, in the case of plateletlysates freezing and rethawing resulted in a significant loss of FXIII-A. FXIII-A concentrations measured in the plasmasamples of healthy individuals and patients correlated well with the concentrations of complexed plasma FXIII ZA2B2. andwith the results of FXIII activity measurements. A reference range of 46?82 fgrplatelet was established for platelet FXIII-A.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban ELISA Platelet Monoclonal antibodies Factro XIII of blood coagulation egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Immunological Methods. - 258 : 1-2 (2001), p. 127-135. -
További szerzők:	Ajzner Éva (1968-) (laboratóriumi szakorvos) Tóth Katalin Ágnes (1977-) (biokémikus, molekuláris biológus) Kárpáti Levente (1968-) (okleveles vegyész) Muszbek László (1942-) (haematológus, kutató orvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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