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001-es BibID:	BIBFORM043759
Első szerző:	Fári Miklós (kertészmérnök)
Cím:	Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato / Fari M., De Resende G. M., De Melo N. F.
Dátum:	2000
ISSN:	0100-204X
Megjegyzések:	The objective of this work was to evaluate the regeneration capacity of the IPA-5 and IPA-6 Brazilian industrial tomato (Lycopersicon esculentum Mill) cultivars using four compositions of culture media described in the literature and five inoculation methods. A new variation of inoculation, the split cotyledon method, was also tested. The largest frequency of shoot bud formation was 100% in the case of IPA-5 and 65% in the case of IPA-6. To induce shoot elongation, it was necessary to accomplish three subcultures of the explants presenting shoot buds. In the case of IPA-5, the number of the obtained shoots was higher when the induction of shoot buds was accomplished in culture medium containing BAP (2.5 mg L-1) and IAA (0.2 mg L-1) followed by three subcultures on zeatin (0.5 mg L-1) containing medium. Using such protocol, the IPA-5 cultivar produced, on the average, 5.45 elongated shoots through the split cotyledon method. That capacity exceeded significantly the trimmed cotyledon method, which produced 4.4 elongated shoots per explant. In the case of IPA-6, the best combination of culture medium and inoculation method produced 0.87 elongated shoot per explant. The elongated shoots were rooted and transferred to greenhouse.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Pesquisa agropecuária brasileira. - 35 : 8 (2000), p. 1523-1529. -
További szerzők:	De Resende, G. M. De Melo, N. F.
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