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001-es BibID:	BIBFORM044316
035-os BibID:	PMID:11522014
Első szerző:	Ulrichts, Hans
Cím:	Selection of phages that inhibit vWF interaction with collagen under both static and flow conditions / H. Ulrichts, H. Depraetere, J. Harsfalvi, H. Deckmyn
Dátum:	2001
ISSN:	0340-6245
Megjegyzések:	Phages from a pentadecamer phage display library were selected for binding to vWF by affinity panning. Bound phages were selectively eluted with human collagen type I. After the third round of panning 95% of individual phage clones bound to vWF. The B8-phage inhibited the binding of collagen to vWF with an IC50 of 0.6 x 10(10) phages/ml, and of vWF to collagen with an IC50 of 1.0 x 10(10) phages/ml at 0.5 microg/ml vWF. Under flow conditions, 1.5 x 10(11) B8-phage/ml nearly completely inhibited platelet deposition on a human collagen type I coated surface at a shear rate of 1200 s(-1), while phages without an insert had no effect. The peptide corresponding to the one displayed on the B8-phage competed with the phage for binding to vWF with an IC50 of 30 microg/ml (16 microM). The peptide furthermore inhibited vWF-binding to collagen with a maximum of 40% at a concentration of 1.25 mg/ml (650 microM), higher concentrations of peptide could not improve this. We thus have selected phages that are potent vWF-binders and that can be used as tools to detect vWF, to inhibit vWF-collagen interaction and to further analyse the role of vWF-collagen binding
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban vWF-collagen vWF collagen
Megjelenés:	Thrombosis and Haemostasis. - 86 : 2 (2001), p. 630-635. -
További szerzők:	Depraetere, H. Hársfalvi Jolán (1949-) (klinikai biokémikus) Deckmyn, Hans
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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