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001-es BibID:	BIBFORM046289
Első szerző:	Bacsó Zsolt (biofizikus)
Cím:	A photobleaching energy transfer analysis of CD8MHC-I and LFA-1ICAM-1 interactions in CTL-target cell conjugates / Bacsó Zsolt, Bene László, Bodnár Andrea, Matkó János, Damjanovich Sándor
Dátum:	1996
ISSN:	0165-2478
Megjegyzések:	The photobleaching energy transfer (pbFRET) technique is a fluorescence method to measure proximity relationships between molecules, especially cell surface proteins, labeled with fluorophore-conjugated monoclonal antibodies, on a pixel-by-pixel base using digital imaging microscopy. This technique enables analysis of inter- and intramolecular proximities at cell surfaces at physiological conditions. We have developed a pbFRET approach to measure intercellular proximities in order to access spatial organization of interacting proteins in the contact region of two 'communicating' cells. Two examples, as possible application areas of this approach, are presented here: interaction between CD8 and MHC-I molecules in point contacts and interaction between LFA-1 and ICAM-1 molecules in focal contacts of CTL-target conjugates. The geometry of these protein contacts based on our resonance energy transfer (RET) data is consistent with the observed blocking effects of monoclonal antibodies (directed against the interacting proteins) on the cytolytic activity of CTLs and suggest a critical role for CD8beta-subunit in signal transmission in peripheral T-lymphocytes
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Immunology Letters. - 54 : 2-3 (1996), p. 151-156. -
További szerzők:	Bene László (1963-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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