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001-es BibID:	BIBFORM046393
Első szerző:	Duvic, M.
Cím:	Keratinocyte transglutaminase expression varies in squamous cell carcinomas / Duvic, M., Nelson, D. C., Annarella, M., Cho, M., Esgleyes-Ribot, T., Remenyik, E., Ulmer, R., Rapini, R. P., Sacks, P. G., Clayman, G. L.
Dátum:	1994
Megjegyzések:	Type I transglutaminase (TGase I, keratinocyte or particulate transglutaminase) is a 92-kilodalton (kDa) protein expressed in abundance in cultured keratinocytes and in the hyperproliferative skin disorder psoriasis. To determine the expression of TGase I protein and mRNA, we studied tissue and established squamous carcinoma lines derived from different sources. Immunohistochemistry and Western blotting were used to detect TGase I protein with the B.C1 mouse monoclonal antibody. Only well-differentiated, skin-derived squamous carcinomas stained for TGase I. However, a precocious pattern of expression was seen overlying less-differentiated tumors. Compared to cultured human keratinocytes, squamous cell carcinoma (SCC) had many times less to 7.8 times more TGase I protein, greatest in the two most differentiated tumor lines 14-83 and ME-180. TGase I mRNA levels ranged from 0.010 to 0.00004 pg/microgram total RNA by reverse transcriptase-polymerase chain reaction using an internal standard. Protein expression correlated with mRNA levels in most SCC lines. When a human TGase I promoter was isolated and used to study genomic DNA, SCC1-83 was shown to have unique restriction enzyme fragments, including one indicative of methylation differences, also present within DNA from the KB line. These studies suggest that transcriptional control of TGase I gene expression in squamous carcinomas may be influenced both by cis elements in the promoter and by the degree of tumor squamous differentiation.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	The Journal of Investigative Dermatology. - 102 : 4 (1994), p. 462-469. -
További szerzők:	Nelson, D. C. Annarella, M. Cho, M. Esgleyes-Ribot, T. Remenyik Éva (1956-) (bőrgyógyász) Ulmer, R. Rapini, R. P. Sacks, P. G. Clayman, G. L.
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