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001-es BibID:	BIBFORM047649
Első szerző:	Bácsi Attila (immunológus)
Cím:	Colostrinin decreases spontaneous and induced mutation frequencies at the hprt locus in Chinese hamster V79 cells / Attila Bacsi, Leopoldo Aguilera-Aguirre, Peter German, Marian L. Kruzel, Istvan Boldogh
Dátum:	2006
Megjegyzések:	ColostrininTM (CLN), a uniform mixture of lowmolecular weight, proline-rich polypeptides, induces neurite outgrowth of pheochromocytoma cells, extends the lifespan of diploid fibroblast cells, inhibits beta amyloid-induced apoptosis and resulted in improved cognitive function when administered to Alzheimer's patients. Here we investigated CLN's antimutagenic activity in cells stressed oxidatively or exposed to chemical or physical agents. Our data show that CLN did not alter cell cycle kinetics and cloning efficiency, while it inhibited the development of spontaneous mutations at the coding region of the hypoxanthine phosphoribosyl-transferase (hprt) gene in Chinese hamster V79 cells. In a dosedependent manner, CLN lowered reactive oxygen species (ROS)-induced frequency of cells resistant to 6-thioguanine (6-TG) to nearly background level. Likewise, CLN decreased the frequency of methyl methanesulfonate- or mitomycin C-induced mutations in V79 cells. Notably, CLN (at 100, 250, and 500 ng per ml concentrations) decreased UVAinduced mutation frequency, while only the highest dose of CLN also decreased significantly the number of UVB-induced 6-TG-resistant mutant cells. Similar results were obtained using cell cultures of human origin. Overall, our data show that CLN significantly lowers the mutation frequency that develops spontaneously or is induced by ROS, chemical and physical agents. CLN itself has no mutagenic activity. Therefore, CLN may be used in human therapies systemically and/or locally for the prevention of diseases associated with sequence alterations in genomic and mitochondrial DNA.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban colostrinin mutation frequency oxidative stress genotoxic chemical and physical agents
Megjelenés:	Journal of Experimental Therapeutics and Oncology 5 : 4 (2006), p. 249-259. -
További szerzők:	Aguilera-Aguirre, Leopoldo Germán Péter (gyermekgyógyász) Kruzel, Marian L. Boldogh István
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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