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001-es BibID:	BIBFORM048029
Első szerző:	Miszti-Blasius Kornél (laboratóriumi szakorvos)
Cím:	P-selectin glycoprotein ligand-1 deficiency augments G-CSF induced myeloid cell mobilization / Kornél Miszti-Blasius, Szabolcs Felszeghy, Csongor Kiss, Ilona Benkő, Krisztina Géresi, Attila Megyeri, Zsuzsanna Hevessy, János Kappelmayer
Dátum:	2014
Megjegyzések:	The effect of G-CSF was investigated in P-selectin Glycoprotein Ligand-1 (PSGL-1) deficient (PSGL-1-/-) and wild-type (PSGL-1+/+) mice to establish the role of this mucin in myeloid cell mobilization. G-CSF activates tissue proteases that cleave adhesion molecules, thus enhances the mobilization of myeloid cells and haematopoietic stem cells. Cytopenia was induced with a single dose of cyclophosphamide. In PSGL-1-/- animals, we observed a delayed extravasation of mature myeloid cells from the peripheral vessels into the tissue compartments and their faster mobilization from the bone marrow. Subsequently, animals received G-CSF twice a day for four days. Neutrophil and monocyte counts increased upon completion of G-CSF treatment and both values were significantly higher in PSGL-1-/- mice; 47.7 G/L vs. 28.3 G/L for neutrophils and 4.1 G/L vs. 2.0 G/L for monocytes. The ratio of atypical myeloid cells was also elevated. Analyzing the causes of the above differences, we identified a 4-fold increase in the colony forming unit (CFU-GM) counts of the peripheral blood in PSGL-1-/- mice, compared to wild type animals. A significantly elevated number of CFU-GM was detected also in the femurs of PSGL-1-/- mice, 4 and 5 days after cyclophosphamide treatment and these values paralleled with the elevation of CD34+/CD117+ stem cell counts in the peripheral blood. Our data suggest, that in the absence of PSGL-1, G-CSF was more potent in elevating absolute myeloid cell numbers by acting on cell release from the bone marrow, maturation from circulating precursor cells in the peripheral blood and prolonged retainment in the circulation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban P-selectin glycoprotein ligand-1 G-CSF Myeloid cells Colony-forming units Stem cells Mobilization
Megjelenés:	Naunyn-Schmiedeberg's Archives of Pharmacology. - 387 : 2 (2014), p. 109-118. -
További szerzők:	Felszeghy Szabolcs Béla (1972-) (fogorvos, anatómus, kötőszövetbiológus) Kiss Csongor (1956-) (hematológus, onkológus) Benkő Ilona (1954-) (orvos, farmakológus) Géresi Krisztina (1985-) (molekuláris biológus) Megyeri Attila (1968-) (orvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Pályázati támogatás:	TÁMOP 4.2.4. A/2-11-1-2012-0001 TÁMOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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