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001-es BibID:	BIBFORM048969
Első szerző:	Szállási Zoltán
Cím:	Effect of enzymic (collagenase) harvesting on the intracellular Na+/K+ ratio of Swiss/3T3 cells as revealed by X-ray microanalysis / Z. Szállási, Á. Szállási, F. Boján, I. Zs.-Nagy
Dátum:	1988
ISSN:	0021-9533
Megjegyzések:	Swiss/3T3 cell cultures were harvested with 0.05% collagenase and after centrifugation the pellet was prepared by the freeze-fracture/freeze-drying (FFFD) method for bulk-specimen X-ray microanalysis. Time-dependent variations in the intracellular monovalent elemental concentrations (Na+, K+ and Cl-) as well as of the Na+/K+ ratio were followed for 120 min subsequent to harvesting. The quantitative measurements revealed a very considerable increase in the intracellular Na+ and Cl- accompanied by a decrease in the K+ concentration as soon as 5 min after harvesting. The Na+/K+ ratio had increased by this time to about 1.5 on average. These changes indicate a sustained depolarization of the cell membrane. During the first 60 min this depolarization tended to normalize as demonstrated by an exponential decrease in the intracellular Na+ and Cl- and an increase in the K+ content involving a decrease in the Na+/K+ ratio. The total intracellular monovalent ion concentration remained almost constant during this post-harvesting period. These results suggest that harvesting represents a serious depolarizing stimulus to the cells, the consequences of which are restored only after 1-2h. These alterations should be taken into consideration during various experimental designs when using anchorage-dependent cell cultures.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban X-ray Na+/K+ Performance Measures transfusion indications single-unit policy
Megjelenés:	Journal of Cell Science. - 30 : Pt. 1 (1988), p. 99-104. -
További szerzők:	Szállási Árpád (1960-) (pathológus) Boján Ferenc (1946-1997) (orvos) Zs. Nagy Imre
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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