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001-es BibID:	BIBFORM049156
035-os BibID:	PMID:23282644
Első szerző:	Almássy János (élettanász, biológus, angol-magyar szakfordító)
Cím:	Investigating ion channel distribution using a combination of spatially limited photolysis, Ca2+ imaging, and patch clamp recording / Janos Almassy, David I. Yule
Dátum:	2013
Megjegyzések:	The production of saliva by parotid acinar cells is stimulated by Ca2+ activation of Cl? and K+ channels located in the apical plasma membrane of these polarized cells. Here, we utilize a combination of spatially limited flash photolysis, Ca2+ imaging, and electrophysiological recording to investigate the distinct distribution of Ca2+-dependent ion channels in the plasma membrane (PM) of enzymatically isolated murine parotid acinar cells. In these experiments, the aim of photolysis is to selectively target and modify the activity of ion channels, thereby revealing membrane-domain-specific differences in distribution. Specifically, the relative distribution of channels to either apical or basal PM can be investigated. Since there is substantial evidence that Ca2+-dependent Cl? channels are exclusively localized to the apical membrane of acinar cells, this provides an important electrophysiologicalverification that a particular membrane has been specifically targeted.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban human myofibroblast photolysis patch clamp
Megjelenés:	Cold Spring Harbor Protocols 1 (2013), p. 1-6. -
További szerzők:	Yule, David I.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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