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001-es BibID:	BIBFORM049404
Első szerző:	Csernoch László (élettanász)
Cím:	Surface charge distribution commands the maurocacline effect on the ryanodine receptor / L. Csernoch, M. Ronjat, J. M. Sabatier, Cs. Szegedi, I. Jóna
Dátum:	2004
Megjegyzések:	Maurocalcine (MCa) ? isolated from the venom of the Scorpio Maurus Palmatus ? has been described as an extremely potent modulator of the sarcoplasmic reticulum calcium release channel (CRC/RyR). Using single channel bilayer electrophysiology and confocal laser scanning microscopy we have shown that MCa is capable of altering the open probability and the gating characteristics of CRC/RyR by inducing long lasting subconductance states (LLSS). LLSS can last for several seconds. In parallel MCa induces complex event of calcium release in line-scan images. Using mutants of MCa we have demonstrated that the surface charge and/or charge distribution is the most important single parameter of the MCa effect. Altering even a single amino acid which destroys the positively charged area overwhelmingly influences the effect of the toxin on CRC/RyR. The effectiveness of the mutants is roughly proportionally with the real distance from the critical 24 residue. Their effects on the isolated channel and on the calcium release events were between those of the wild type and the ineffective Arg24Ala mutant.
Tárgyszavak:	Természettudományok Biológiai tudományok idézhető absztrakt calcium release
Megjelenés:	Journal of Muscle Research and Cell Motility. - 25 (2004), p. 257. -
További szerzők:	Ronjat, Michel Sabatier, Jean Marc Szegedi Csaba Jóna István (1948-) (élettanász, fizikus)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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