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001-es BibID:	BIBFORM049406
Első szerző:	Lukács Balázs (élettanász)
Cím:	Effects of natural derivatives of phenol on calcium transport of sarcoplasmic reticulum / B. Lukacs, G. Nagy, N. Dobrosi, I. Gherasim, I. Jona
Dátum:	2005
Megjegyzések:	The effect of natural derivatives of phenol was investigated on sarcoplasmic reticulum calcium pump (SERCA) isolated from rabbit and calcium release channel (RyR1) isolated from normal and malignant hyperthermia susceptible pig (MHS) skeletal muscle. SERCA activity was determined using ♭coupled enzyme method', the decrease of NADH concentration was followed photometrically. The effects of some of these agents on the calcium release and on the 3H ryanodine binding were investigated too. Thymol and trans-anethole inhibited the Ca2+ATPase with a Kd of 224?10 lM and 168?10 lM, respectively. The vanillin, orthovanillin, 4-methyl-2-nitrophenol, meta-anisaldehyde and cineole exerted only unspecific inhibition. In calcium release measurements, the thymol and the carvacrol activated the channel with a Kd of 158?16 lM and 211?55 lM, respectively. In binding assay experiments the thymol activated both the MHS and wild type RyR1. The MHS type RyR1 showed lower Kd to ryanodine and could bind more ryanodine than the normal type RyR1both in control and in the presence of thymol.
Tárgyszavak:	Természettudományok Biológiai tudományok idézhető absztrakt calcium release
Megjelenés:	Journal of Muscle Research and Cell Motility. - 26 (2005), p. 61. -
További szerzők:	Nagy G. Dobrosi Nóra (1981-) (molekuláris biológus) Gherasim, Iuliana Jóna István (1948-) (élettanász, fizikus)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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