Találati lista | Tudóstér

001-es BibID:	BIBFORM050040
Első szerző:	Bhattacharya, Anirban
Cím:	Potentiation of inhibitory synaptic transmission by extracellular ATP in rat suprachiasmatic nuclei / Anirban Bhattacharya, Vojtech Vavra, Irena Svobodova, Zdena Bendova, Gyorgy Vereb, Hana Zemkova
Dátum:	2013
Megjegyzések:	The hypothalamic suprachiasmatic nuclei (SCN), the circadian master clock in mammals, releases ATP in a rhythm, but the role of extracellular ATP in the SCN is still unknown. In this study, we examined the expression and function of ATP-gated P2X receptors (P2XRs) in the SCN neurons of slices isolated from the brain of 16- to 20-day-old rats. Quantitative RT-PCR showed that the SCN contains mRNA for P2X 1-7 receptors and several G-protein-coupled P2Y receptors. Among the P2XR subunits, the P2X2 > P2X7 > P2X4 mRNAs were the most abundant. Whole-cell patch-clamp recordings from SCN neurons revealed that extracellular ATP application increased the frequency of spontaneous GABAergic IPSCs without changes in their amplitudes. The effect of ATP appears to be mediated by presynaptic P2X2Rs because ATPgammaS and 2MeS-ATP mimics, while the P2XR antagonist PPADS blocks, the observed enhancement of the frequency of GABA currents. There were significant differences between two SCN regions in that the effect of ATP was higher in the ventrolateral subdivision, which is densely innervated from outside the SCN. Little evidence was found for the presence of P2XR channels in somata of SCN neurons as P2X2R immunoreactivity colocalized with synapsin and ATP-induced current was observed in only 7% of cells. In fura-2 AM-loaded slices, BzATP as well as ADP stimulated intracellular Ca(2+) increase, indicating that the SCN cells express functional P2X7 and P2Y receptors. Our data suggest that ATP activates presynaptic P2X2Rs to regulate inhibitory synaptic transmission within the SCN and that this effect varies between regions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adenosine Adenosine Triphosphate Animals Animals,Newborn article Biophysical Phenomena Brain Calcium Cells Cells,Cultured cytology Dose-Response Relationship,Drug drug effects Excitatory Amino Acid Antagonists EXPRESSION gamma-Aminobutyric Acid Gene Expression Regulation genetics In Vitro Male metabolism Neural Inhibition Neurons Patch-Clamp Techniques pharmacology physiology Platelet Aggregation Platelet Aggregation Inhibitors Purinergic Agents Rats Rats,Wistar Receptors,Purinergic P2X Research Research Support rna RNA,Messenger Sodium Sodium Channel Blockers Support Suprachiasmatic Nucleus Synaptic Potentials Synaptic Transmission Tetrodotoxin
Megjelenés:	The Journal of Neuroscience. - 33 : 18 (2013), p. 8035-8044. -
További szerzők:	Vavra, Vojtech Svobodova, Irena Bendova, Zdena Vereb György (1965-) (biofizikus, orvos) Zemkova, Hana
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: