Találati lista | Tudóstér

001-es BibID:	BIBFORM060997
035-os BibID:	(WOS)000345980600003 (Scopus)84913614417
Első szerző:	Papp László Attila (genetikus)
Cím:	Optimal conditions for mycelial growth of Schizosaccharomyces japonicus cells in liquid medium : it enables the molecular investigation of dimorphism / László Papp, Matthias Sipiczki, Imre J. Holb, Ida Miklós
Dátum:	2014
ISSN:	0749-503X
Megjegyzések:	The non-pathogenic dimorphic fission yeast, Schizosaccharomyces japonicus, could be a suitable model organism for investigation of the genetic background of mycelial growth, as it has a haploid chromosome set and its genome is sequenced. Since earlier results have suggested that its morphological transition required solid substrates, but molecular biological experiments would require hyphae production in a liquid medium, we wanted to find circumstances which would enable hyphae production in liquid media. Several external conditions were investigated, but the strongest inducer was fetal bovine serum (FBS). Its positive effect could be hampered by heat and was dependent on pH, temperature and concentration of the serum. Other protein-containing compounds, such as peptone and bovine serum albumin or amino acids, proved to be ineffective or weak. Generally, the uninduced and induced mycelial growth of Sz. japonicus could be improved by lower external pH and higher temperature.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Schizosaccharomyces japonicus
Megjelenés:	Yeast. - 31 : 12 (2014), 475-482. -
További szerzők:	Sipiczki Mátyás (1948-) (biológus) Holb Imre (1973-) (agrármérnök) Miklós Ida (1962-) (biológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI Szerző által megadott URL
Borító:
Saját polcon: