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001-es BibID:	BIBFORM062408
Első szerző:	Oláh Tamás (élettanász)
Cím:	CB1 cannabinoid receptors are involved in the regulation of excitation-contraction coupling in mammalian skeletal muscle / Oláh Tamás, Bodnár Dóra, Tóth Adrienn, Fodor János, Kovács Adrienn, Farkas Anna, Nádró Bíborka, Szentesi Péter, Csernoch László
Dátum:	2015
Megjegyzések:	The presence of CB1 cannabinoid receptors (CB1R) has been shown in skeletal muscle, but it is yet to be cleared whether they have any significance in the regulation of contractions. CB1-knockout (CB1-KO) mice showed hypoactivity, however, it is questionable whether this was solely due to effects on the central nervous system or impairment of muscle function also contributes. Our aim was to investigate the role of CB1R in mammalian skeletal muscle, and the effects of cannabinoid drugs on Ca2+ transients. Running ability of control and CB1-KO mice was studied by activity-wheel-tests while in vivo muscle force of the animals was measured by grip-tests and hang-tests. Ca2+ transients evoked by KCl-depolarization in the presence and absence of cannabinoid agonists were investigated on flexor digitorum brevis (FDB) fibers of control and CB1-KO mice. CB1-KO mice performed worse as compared to control in all behavior tests applied. In contrast, depolarization-evoked Ca2+ transients were significantly higher in FDB fibers isolated from CB1-KO mice (848 ? 98 nM, n = 47) compared to control (376 ? 60 nM, n = 32, p\0.01). When KCl-evoked Ca2+ transients were repeated on control FDB fibers in the absence and presence of the CB1 agonist WIN55,212 (WIN), the transients after WIN treatment were significantly smaller (44 ? 7 % of the first transient, n = 27) than in untreated (79 ? 5 % of the first transient, n = 32, p\0.01) fibers. Our observations suggest that CB1R-mediated signaling contributes to the regulation of excitation?contraction coupling and skeletal muscle contractions. Nevertheless, the effects mediated by the absence of CB1R in the central nervous system on the inferior muscle performance of CB1-KO mice in vivo cannot be ruled out. These results can contribute to the identification of the side effects of medically used cannabinoid drugs on skeletal muscle.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt Skeletal muscle CB1 cannabinoid receptor Excitation?contraction coupling
Megjelenés:	Journal of muscle research and cell motility. - 36 (2015), p. 129. -
További szerzők:	Bodnár Dóra (1987-) (molekuláris biológus) Tóth Adrienn (1988-) (molekuláris biológus, élettanász) Fodor János (1973-) (élettanász, biotechnológus) Kovács Adrienn (1989-) (molekuláris biológus) Farkas Anna (1983-) Nádró Bíborka (1992-) (általános orvos) Szentesi Péter (1967-) (élettanász) Csernoch László (1961-) (élettanász)
Pályázati támogatás:	TÁMOP-4.2.4.A/2-11-1-2012-0001 TÁMOP
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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