Találati lista | Tudóstér

001-es BibID:	BIBFORM064998
Első szerző:	Dózsa Anikó (Ph.D hallgató, orvos)
Cím:	PPAR[gamma] has different expression and signaling pattern in normal and pathologic sebaceous glands / A. Dozsa, B. I. Tóth, T. Bíró, E. Remenyik, B. Dezso, C. C. Zouboulis, L. Nagy
Dátum:	2009
Megjegyzések:	Peroxisome proliferation activated receptor gamma (PPAR?) has been implicated in lipid metabolismand also in infl ammation. PPAR? is expressed in lipid rich human sebocytes and it appears to haveroles in their functions. The details of PPAR?-regulated lipid metabolism in sebocytes are, however,not well understood. Therefore our aim was to characterize the expression patterns of PPAR?, itsheterodimeric partner RXR and target genes in normal and pathologic sebaceous glands in tissuesand in the SZ95 sebocytes . Therefore we applied formaldehyde fi xed parraffi n embedded skinsamples of patients with sebaceous hyperplasia, sebaceous carcinoma for immunohistochemistry.From fresh frozen skin samples, we laser microdissected normal sebaceous glands and isolatedRNA for RT-PCR. We studied function of PPAR? molecule in SZ95 sebocyte culture by addingPPAR? agonist rosiglitazone (RSG), RXRalfa agonist LG268 or arachidonic acid (AA). Viabilityof the cells was measured by MTT test. Nile red staining and quantitative fl uorimetric analysiswere used to detect changes in polar and neutral lipid content levels as a differentiation markerof sebocytes. We found that PPAR? and RXR? proteins are present in normal and hyperplasticsebaceous glands, immortalized SZ95 cell line, but PPAR? is barely expressed in sebaceouscarcinoma. We also demonstrated that mRNA of PPAR? and its lipid-metabolism associatedtarget genes, ADRP and PGAR were present in both models, but only sebaceous glands in situexpressed FABP4. Target genes responded to activation or inhibition of PPAR? and RXR? inSZ95 sebocytes. These data suggest that PPAR? as a transcription factor is likely to play a role innormal and pathological sebaceous gland biology, thus possibly serving as a relevant target forfurther investigations in sebaceous gland-associated dermatoses and a potential target of therapy.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt PPAR gamma sebocyte lipid
Megjelenés:	Journal of Investigative Dermatology. - 129 : Suppl. 2 (2009), p. S54. -
További szerzők:	Tóth István Balázs (1978-) (élettanász) Bíró Tamás (1968-) (élettanász) (absztraktok) Remenyik Éva (1956-) (bőrgyógyász) Dezső Balázs (1951-) (pathológus) Zouboulis, Christos C. (1960-) (bőrgyógyász) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: