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001-es BibID:	BIBFORM065022
Első szerző:	Kovács Csilla (élelmiszerbiztonsági és minőségi mérnök)
Cím:	Molecular characterization of GTD endophytic and pathogenic fungi / Csilla Kovács, Erzsébet Sándor, Zoltán Bihari, Ferenc Peles
Dátum:	2014
ISSN:	0133-8455
Megjegyzések:	Grapevine trunk diseases are amongst the most important diseases in vineyards worldwide and in Hungary. GTD are caused by several pathogenic fungi (e.g. Phaeoacremonium aleophilum, Phaeomoniella chlamydospora, Eutypa lata, Fomitiporia mediterranea, Diplodia seriata).There is very limited information about the pathogens in Tokaj wine region nowadays.Pathogenic fungi were isolated from 207 symptomatic grape trunks from the Tokaj wine region in 2013. The samples with foliar symptoms originated from four areas: Szemere (Degenfeld); Dorgó (Disznókő); Szarvas (Kereskedőház), and Várhegy (Patrícius) vineyards. The woody tissue samples were cut in a small part, and were disinfected before putting on malt extract agar medium and incubated at room temperature for 3-8 days. MagNaLyser (Roche) was used for disruption, and isolation was carried with NucleoSpin Plant II. The ITS4 and ITS5 primers were used for PCR amplification of the internal transcribed spacer regions. Purified amplicons were sequenced by Mycrosinth, Austria. The sequences were aligned with deponated reference sequences with Clustal X program, and manually corrected with GeneDoc. Phylogenetic analyses were performed with MEGA 5.05366 pure fungal cultures were isolated and identified till now. The majority of the isolates (56%) were determined as Diplodia seriata (Botryosphaeriaceae). Other fungi, like Fusarium sp. (13.9%), Alternaria sp. (9.3%), Aspergillus sp. (7.9%), Mucor sp. (7.1%), Diaporthe sp. (3.3%), Trichoderma sp. (1.4%), Epicoccum sp. (0.5%), Penicillium (0.3%) and Xanthomendoza sp. (0.3%) were also identified from grapevine trunk samples.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idézhető absztrakt endophytic fungi pathogenic fungi GTD
Megjelenés:	Annual Meeting of Hungarian Biochemical Society Final Programme and Abstract Book. Biokémia 38 : 3 (2014), p. 74. -
További szerzők:	Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Bihari Zoltán (1967-) (biológus) Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Pályázati támogatás:	TÁMOP-4.2.4.A/2-11-1-2012-0001 TÁMOP COST FA1303 Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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