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001-es BibID:	BIBFORM065195
Első szerző:	Kehler, Lars
Cím:	Elevated hsa-miR-99a levels in maternal plasma may indicate congenital heart defects / Lars Kehler, Orsolya Biro, Levente Lazar, Janos Rigo Jr., Balint Nagy
Dátum:	2015
ISSN:	2049-9434 2049-9442
Megjegyzések:	The current standard for prenatal screening is mostly based on biochemical marker tests and the use of ultrasonography. There is no secure stand-alone screening marker for congenital heart defects (CHDs). MicroRNAs (miRNAs) that are associated with cardiogenesis enter the maternal peripheral bloodstream during pregnancy and allow non-invasive prenatal testing (NIPT). The present study investigated the plasma expression profile of fetal hsa-miR-99a in maternal blood. Peripheral blood samples were collected from 39 pregnant patients, comprising 22 with CHD-positive fetuses and 17 with CHD-free controls. miRNAs were isolated from the maternal serum and reverse transcription-quantitative polymerase chain reaction was carried out to determine the expression of hsa-miR-99a. While the miRNA concentrations were almost identical among the affected and control groups (5.54 vs. 6.40 ng/?l), significantly upregulated hsa-miR-99a levels were identified in the affected group (1.78?10?2?3.53?10?2 vs. 1.09?10?3?3.55?10?3 ng/?l, P=0.038). In conclusion, according to the present study, hsa-miR-99a is involved in cardiac malformation and may serve as a biomarker during fetal development, and therefore presents as a candidate for monitoring cardiomyogenesis and potential use as a NIPT-biomarker for fetal CHD.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban microRNA congenital heart defects non-invasive prenatal diagnosis fetal diagnosis
Megjelenés:	Biomedical Reports. - 3 (2015), p. 869-873. -
További szerzők:	Biró Orsolya (molekuláris biológus) Lázár Levente (szülész-nőgyógyász) Rigó János (1928-) (sebész, ortopéd traumatológus) Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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