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001-es BibID:	BIBFORM065710
Első szerző:	Solti Ádám
Cím:	Does a voltage-sensitive outer envelope transport mechanism contributes to the chloroplast iron uptake? / Ádám Solti, Krisztina Kovács, Brigitta Müller, Saúl Vázquez, Éva Hamar, Hong Diep Pham, Brigitta Tóth, Javier Abadía, Ferenc Fodor
Dátum:	2016
ISSN:	0032-0935
Megjegyzések:	Although iron (Fe) plays a crucial role in chloroplast physiology, only few pieces ofinformation are available on the mechanisms of chloroplast Fe acquisition.Here, the effect of inorganic salts on the Fe uptake of intact chloroplasts was tested,assessing Fe and transition metal uptake using bathophenantroline-basedspectrophotometric detection and plasma emission coupled mass spectrometry, respectively. The microenvironment of Fe was studied by Mössbauer spectroscopy.Transition metal cations (Cd2+, Zn2+, Mn2+) enhanced, whereas oxoanions (NO3-,SO42- and BO33-) reduced the chloroplast Fe uptake. The effect was insensitive to diuron (DCMU), an inhibitor of chloroplast inner envelope associated Fe uptake. Theinorganic salts affected neither Fe forms in the uptake assay buffer nor thoseincorporated into the chloroplasts. The significantly lower Zn and Mn uptake comparedto that of Fe indicates that different mechanisms/transporters are involved in theiracquisition.The enhancing effect of transition metals on chloroplast Fe uptake is likely related toouter envelope-associated processes since divalent metal cations are known to inhibitFe2+ transport across the inner envelope. Thus, a voltage-dependent step is proposedto play a role in Fe uptake through the chloroplast outer envelope on the basis of thecontrasting effects of transition metal cations and oxoaninons.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban Chloroplast Envelope membrane Iron metabolism Mössbauer spectrosopy
Megjelenés:	Planta 244 : 6 (2016), p. 1303-1313. -
További szerzők:	Kovács Krisztina Müller Brigitta Vázquez, Saúl Hamar Éva Pham Hong Diep Tóth Brigitta (1984-) (környezetgazdálkodási agrármérnök) Abadía, Javier Fodor Ferenc
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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