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001-es BibID:	BIBFORM066224
Első szerző:	Németh Zoltán (biomérnök)
Cím:	Growth-phase sterigmatocystin formation on lactose is mediated via low specific growth rates in Aspergillus nidulans / Németh Zoltán, Molnár Ákos P., Fejes Balázs, Novák Levente, Karaffa Levente, Nancy P. Keller, Fekete Erzsébet
Dátum:	2016
ISSN:	2072-6651
Megjegyzések:	Seed contamination with polyketide mycotoxins such as sterigmatocystin (ST) produced by Aspergilli is a worldwide issue. The ST biosynthetic pathway is well-characterized in A. nidulans, but regulatory aspects related to the carbon source are still enigmatic. This is particularly true for lactose, inasmuch as some ST production mutant strains still synthesize ST on lactose but not on other carbon substrates. Here, kinetic data revealed that on D-glucose, ST forms only after the sugar is depleted from the medium, while on lactose, ST appears when most of the carbon source is still available. Biomass-specified ST production on lactose was significantly higher than on D-glucose, suggesting that ST formation may either be mediated by a carbon catabolite regulatory mechanism, or induced by low specific growth rates attainable on lactose. These hypotheses were tested by D-glucose limited chemostat-type continuous fermentations. No ST formed at high growth rate, while low growth rate led to the formation of 0.4 mg L-1 ST. Similar results were obtained with a CreA mutant strain. We concluded that low specific growth rates may be the primary cause of mid-growth ST formation on lactose in A. nidulans, and that carbon utilization rates likely play a general regulatory role during biosynthesis.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Aspergillus nidulans sterigmatocystin lactose D-glucose CreA growth rate
Megjelenés:	Toxins 8 : 12 (2016), p. 354. -
További szerzők:	Molnár Ákos Péter (1988-) (okleveles biomérnök) Fejes Balázs Novák Levente (1967-) (biológus) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár) Keller, Nancy P. Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár)
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