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001-es BibID:	BIBFORM068267
035-os BibID:	(cikkazonosító)321 (Scopus)85016596205 (WOS)000395567000001
Első szerző:	Bene Krisztián (Biológus)
Cím:	Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors / Bene P. Krisztián, Kavanaugh Devon W., Leclaire Charlotte, Gunning Allan P., MacKenzie Donald A., Wittmann Alexandra, Young Ian D., Kawasaki Norihito, Rajnavolgyi Eva, Juge Nathalie
Dátum:	2017
ISSN:	1664-302X
Megjegyzések:	The vertebrate gut symbiont Lactobacillus reuteri exhibits strain-specific adhesion and health-promoting properties. Here, we investigated the role of the mucus adhesins, CmbA and MUB, upon interaction of L. reuteri ATCC PTA 6475 and ATCC 53608strains with human monocyte-derived dendritic cells (moDCs). We showed that mucus adhesins increased the capacity of L. reuteri strains to interact with moDCs and promoted phagocytosis. Our data also indicated that mucus adhesins mediate antiand pro-inflammatory effects by the induction of interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-a), IL-1b, IL-6, and IL-12 cytokines. L. reuteri ATCC PTA 6475 and ATCC 53608 were exclusively able to induce moDC-mediated Th1 and Th17 immune responses. We further showed that purified MUB activates moDCs and induces Th1 polarized immune responses associated with increased IFNg production. MUB appeared to mediate these effects via binding to C-type lectin receptors (CLRs), as shown using cell reporter assays. Blocking moDCs with antibodies against DC-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) or Dectin-2 did not affect the uptake of the MUB-expressing strain, but reduced the production of TNF-a and IL-6 by moDCs significantly, in line with the Th1 polarizing capacity of moDCs.The direct interaction between MUB and CLRs was further confirmed by atomic force spectroscopy. Taken together these data suggest that mucus adhesins expressed at the cell surface of L. reuteri strains may exert immunoregulatory effects in the gut through modulating the Th1-promoting capacity of DCs upon interaction with C-type lectins.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk immunológia mikrobiológia Lactobacillus reuteri dendritikus sejt mikrobióta
Megjelenés:	Frontiers in Microbiology. - 8 (2017), p. 1-16. -
További szerzők:	Kavanaugh, Devon W. Leclaire, Charlotte Gunning, Allan P. MacKenzie, Donald A. Wittmann, Alexandra Young, Ian D. Kawasaki, Norihito Rajnavölgyi Éva (1950-) (immunológus) Juge, Nathalie
Pályázati támogatás:	FP7-KBBE-2007-2A FP7 TÁMOP 4.2.4. A/2-11-1-2012-0001 TÁMOP TÁMOP 4.2.2.A-11/1/KONV-2012-0023 TÁMOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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