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001-es BibID:	BIBFORM068805
Első szerző:	Herczeg Dávid (biológus)
Cím:	Molecular taxonomic identification and determination of hybrid ploidy levels among Europen water frogs (Pelophylax) in Hungary / Dávid Herczeg, Judit Vörös, Michal Benovic, Peter Mikulícek
Dátum:	2015
Megjegyzések:	The western palearctic water frogs in the genus Pelophylax include twelve species with partially overlapping morphology. Some of these species can form hybridogenetic complexes. Such type is the Pelophylax esculentus complex formed by two sexual species: Pelophylax ridibundus (genotype RR), Pelophylax lessonae (genotype LL), and their hybridogenetic hybrid Pelophylax esculentus (genotype LR, LRR or LLR). This study was conducted in the Hortobágy National Park which is the largest contiguous alkaline steppe in Europe covering 80.000 hectares. Altogether 164 water frogs were captured during the years 2012-2014 from three different types of wetland habitats. Due to the high rate of hybridization and partially overlapping morphological features among water frogs we used molecular taxonomic techniques to distinguish between the parental species and the hybrid. This method is based on the PCR products size differences from the amplification of the serum albumin intron-1 (SAI-1) gene fragment. We also used 15 microsatellite loci to distinguish between the three different genotypes in hybrid individuals. Based on fragmentary analysis we detected the presence of all the members of P. esculentus complex. From 164 samples we determined the taxonomic status of 101 P. ridibundus, 1 P. lessonae and 62 P. esculentus individuals. Based on microsatellite analysis we found that only the diploid (LR) genotype was presented in hybrid individuals
Tárgyszavak:	Természettudományok Biológiai tudományok előadáskivonat
Megjelenés:	Programme & Abstracts : XVIII European Congress of Herpetology, 7-12 September 2015, Wroclaw, Poland / Eds. Borczyk B., Ogielska M., Kolenda K., Skawiński T. - p. 47.
További szerzők:	Vörös Judit Benovics, Michal Mikulíček, Peter
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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