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001-es BibID:	BIBFORM069282
Első szerző:	Kurtz, Andreas
Cím:	Mammary Epithelial Cells of Lactating Rats Express Prolactin Messenger Ribonucleic Acid / Andreas Kurtz, Lynn A. Bristol, Béla E. Tóth, Eliane Lazar-Wesley, László Takács, Bálint Kacsóh
Dátum:	1993
ISSN:	0006-3363
Megjegyzések:	The presence of prolactin (PRL) mRNA in the mammary gland, placenta, and pituitary gland of lactating and pregnant ratswas investigated by polymerase chain reaction (PCR). Polyadenylated RNA was prepared from total RNA samples by oligo(dT)-cellulose chromatography, and complementary cDNAs were synthesized. A standardized amount of cDNA from each sample wasused as the template in a Taq PCR under high-stringency conditions. PCR amplified a signal with the predicted size of approximately375 bp in mammary and pituitary glands of lactating and pregnant rats, and in placentae of pregnant rats. This bandspecifically hybridized with a probe overlapping the entire sequence of the mature rat (r) PRL mRNA in Southern blot analysis.When the rPRL-specific primers were used, PCR revealed no signal in the liver or in lactating mammary gland explants culturedin vitro for 48 h, while the same cDNA preparations gave strong signals for R-actin. The viability of the mammary gland explantswas also suggested by their ability to secrete immunoreactive casein in vitro. PRL mRNA was localized in the epithelium of alveoliand ducts of the lactating mammary gland by in situ hybridization. These data provide evidence that the PRL gene is expressedin the mammary gland of pregnant and lactating rats, and suggest that the mammary gland might contribute to PRL in milk byde novo synthesis. Thus, while the placenta is an exogenous source of PRL-like activities for the fetus in utero, the mammarygland might take over this function after birth.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Biology of Reproduction 48 : 5 (1993), p. 1095-1103. -
További szerzők:	Bristol, Lynn A. Tóth E. Béla (1960-) (orvos) Lazar-Wesley, Eliane Takács László (1955-) Kacsóh Bálint
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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