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001-es BibID:	BIBFORM069325
Első szerző:	Szél Ágoston
Cím:	Monoclonal antibody-recognizing cone visual pigment / Á. Szél, L. Takács, É. Monostori, T. Diamantstein, I. Vigh-Teichmann, P. Röhlich
Dátum:	1986
ISSN:	0014-4835
Megjegyzések:	Monoclonal antibodies were raised to a crude photoreceptor-membrane suspension from chicken retinas. Clones producing antibodies against cone outer segments were selected by screening with immunocytochemistry on semithin sections of the retina. One monoclonal antibody, called COS-1, specifically labelled outer segments of double cones and one type of single cones; outer segments of rods and several single cones were not stained. On immunoblots of retinal photoreceptor membranes, this antibody recognized a protein with an apparent molecular mass of 33,000. The visual pigment character of the 33,000 protein was indirectly established by another monoclonal antibody, OS-2, which labelled all outer segments on semithin sections and four bands (33,000-, 36,000-, 38,000-40,000- and a composite band between 66,000-72,000 MW) on immunoblots. Of these, the 36,000- and the 72,000 MW protein bands were identified with an anti-rhodopsin polyclonal antibody as rhodopsin monomer and dimer. Monoclonal antibody OS-2 is assumed therefore to represent an antibody against a common epitope of all visual pigments of the chicken. The monoclonal antibody COS-1 was found to bind to certain cone outer segments of many other vertebrate species as well.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Experimental Eye Research 43 : 6 (1986), p. 871-883. -
További szerzők:	Takács László (1955-) Monostori Éva Diamantstein Tibor Vigh-Teichmann I. Röhlich Pál
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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