Magyar
Toggle navigation
Tudóstér
Magyar
Tudóstér
Keresés
Egyszerű keresés
Összetett keresés
CCL keresés
Egyszerű keresés
Összetett keresés
CCL keresés
Böngészés
Saját polc tartalma
(
0
)
Korábbi keresések
Összesen 1 találat.
#/oldal:
12
36
60
120
Rövid
Hosszú
MARC
Részletezés:
Rendezés:
Szerző növekvő
Szerző csökkenő
Cím növekvő
Cím csökkenő
Dátum növekvő
Dátum csökkenő
1.
001-es BibID:
BIBFORM070542
Első szerző:
Bardóczi Zsuzsanna
Cím:
Glycinergic input to the mouse basal forebrain cholinergic neurons / Bardóczi Z., Pál B., Kőszeghy Á., Wilheim T., Watanabe M., Záborszky L., Liposits Z., Kalló I.
Dátum:
2017
Megjegyzések:
The basal forebrain (BF) receives afferents from brain stem ascending pathways, which has been implicated first by Moruzzi and Magoun (Moruzzi and Magoun, 1949) to induce forebrain activation and cortical arousal/waking behavior; however, it is very little known about how brain stem inhibitory inputs affect cholinergic functions. In the current study, glycine, a major inhibitory neurotransmitter of brain stem neurons, and gliotransmitter of local glial cells, was tested for potential interaction with basal forebrain cholinergic (BFC) neurons in male mice. In the BF, glycine receptor ? subunit-immunoreactive (GlyR?-IR) sites were localized in choline acetyltransferase (ChAT)-IR neurons. Glycine's effect on BFC neurons was demonstrated by bicuculline-resistant, strychnine-sensitive spontaneous inhibitory postsynaptic currents (IPSCs; 0.81 ? 0.25 *10-1 Hz) recorded in whole cell conditions. Potential neuronal, as well as glial sources of glycine were indicated in the extracellular space of cholinergic neurons by glycine transporter 1 and 2 (GLYT1 and 2)-IR processes found in apposition to ChAT-IR cells. Ultrastructural analyses identified synapses of GLYT2-positive axon terminals on ChAT-IR neurons, as well as GLYT1-positive astroglial processes, which were localized in the vicinity of synapses of ChAT-IR neurons. The brain stem raphe magnus was determined to be a major source of glycinergic axons traced retrogradely from the BF. Our results indicate a direct effect of glycine on BFC neurons. Furthermore, the presence of high levels of plasma membrane glycine transporters in the vicinity of cholinergic neurons suggests a tight control of extracellular glycine in the BF.SIGNIFICANCE STATEMENTBFC neurons receive various activating inputs from specific brain stem areas, and channel this information to the cortex via multiple projections. So far very little is known about inhibitory brain stem afferents to the BF. The current study established glycine as a major regulator of BFC neurons by (1) identifying glycinergic neurons in the brain stem projecting to the BF, (2) showing GlyR?-IR sites in ChAT-IR neurons, (3) demonstrating GLYT2-positive axon terminals synapsing on ChAT-IR neurons, and (4) localizing GLYT1-positive astroglial processes in the vicinity of synapses of ChAT-IR neurons. Glycine's effect on BFC neurons was demonstrated by bicuculline-resistant, strychnine-sensitive spontaneous IPSCs recorded in whole cell conditions.
Tárgyszavak:
Természettudományok
Biológiai tudományok
idegen nyelvű folyóiratközlemény külföldi lapban
basal forebrain
glycinergic
Megjelenés:
The Journal of Neuroscience 37 : 39 (2017), p. 9534-9549. -
További szerzők:
Pál Balázs (1975-) (élettanász)
Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász)
Wilheim Tamás
Watanabe, Masahiko
Záborszky László
Liposits Zsolt
Kalló Imre
Pályázati támogatás:
KTIA_13_NAP-A-I/10
Egyéb
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
Rekordok letöltése
1
Corvina könyvtári katalógus v8.2.27
© 2023
Monguz kft.
Minden jog fenntartva.