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001-es BibID:	BIBFORM071072
035-os BibID:	(WOS)000383091900301
Első szerző:	Mihály Johanna (biológus, vegyész)
Cím:	Establishment and optimization of pro-inflammatory model systems in human keratinocytes / Mihaly J., Angyal A., Szilagyi S. B., Tubak V., Soeberdt M., Abels C., Olah A., Biro T.
Dátum:	2016
ISSN:	0022-202X
Megjegyzések:	Keratinocytes were already shown to express various pathogen-associated molecular pattern recognizing receptors, and to produce anti-microbial peptides and pro-inflammatory cytokines, which are important in regulating cutaneous immune processes. Thus, acting on keratinocytes augurs to be a highly efficient and targeted way to alleviate cutaneous inflammation. Therefore, in the current methodological study, we aimed to establish a wide array of easy-to-handle, keratinocyte-based pro-inflammatory model systems, in which efficiency of novel anti-inflammatory compounds can be investigated.The viability of immortalized (HaCaT, HPV-KER) human epidermal keratinocytes was investigated by MTT-assay. Pro-inflammatory response was assessed by measuring the expression (RT-qPCR) and release (ELISA) of selected pro-inflammatory cytokines upon various 3-, 6-, 12- and 24-hr treatments. The revealed optimal duration and dosing of the treatments together with the suggested cell types and read-out parameters are as follows 1. Non-specific irritation/inflammation model: 1 ?M SDS treatment for 24 hrs on HaCaT keratinocytes; recommended endpoint is IL8.2. Contact irritation/inflammation: 1 ?M NiCl2 treatment for 6 hrs on HPV-KERs; recommended read-outs are IL6 and IL8.3. Chemical irritation/inflammation: 300 ?M carvacrol (6 hrs; HPV-KER). Recommended endpoints: IL1?, TNF?, IL6 and IL8.4. Protease induced irritation/inflammation: 10 ?M SLIGRL treatment (3-6 hrs; HaCaT). Recommended endpoints: IL6 and IL8.5. UVB-induced inflammation: 40 mJ/cm2 treatments followed by 6-hr incubation on HPV-KERs; recommended read-outs are IL1?, IL1?, IL6, IL8 and TNF?.6. Poly-(I:C)-induced inflammation: 3-hr treatments on both HPV-KERs and HaCaT keratinocytes; recommended read-outs are IL1?, IL1?, Il6 and IL8.7. Atopic dermatitis-like irritation/inflammation: combined treatment with SEB (100 ng/ml) and TSLP (30 ng/ml) (48-72 hrs; HPV-KER). Read-out parameters: IL1?, TNF?, IL6 and IL8.We could successfully establish various different pro-inflammatory model systems suitable for multifaceted, medium throughput investigation of novel anti-inflammatory compounds.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt folyóiratcikk anti-inflammatory, fluorinated phytocannabinoids pro-inflammatory model systems
Megjelenés:	Journal Of Investigative Dermatology. - 136 : 9 (2016), p. S212. -
További szerzők:	Angyal Ágnes (1987-) (molekuláris biológus) Szilágyi S. B. Tubak Vilmos Soeberdt, Michael Abels, Christoph Oláh Attila (1984-) (élettanász) Bíró Tamás (1968-) (élettanász) (absztraktok)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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