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001-es BibID:	BIBFORM071356
Első szerző:	Nagy Béla Jr. (labordiagnosztikai szakorvos)
Cím:	Serum HE4 is a suitable inflammatory biomarker in cystic fibrosis / B. Nagy Jr., L. Fila, L. A. Clarke, Z. Fejes, P. Antal-Szalmás, J. Kappelmayer, M. D. Amaral, M. Macek Jr., I. Balogh
Dátum:	2016
Megjegyzések:	Serum HE4 is a suitable inflammatory biomarker in cystic fibrosisB. Nagy Jr1, L. Fila2, L.A. Clarke3, Z. Fejes1, P. Antal-Szalmás1, J. Kappelmayer1, M.D. Amaral3, M. Macek Jr4, I. Balogh11Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary, 2Department of Pulmonology,Charles University, 2nd Faculty of Medicine, Motol University Hospital, Prague, Czech Republic, 3University of Lisboa, Faculty of Sciences,BioISI-Biosystems & Integrative Sciences Institute, Lisboa, Portugal, 4Department of Biology and Medical Genetics, Motol UniversityHospital, 2nd Faculty of Medicine, Charles University, Prague, Czech RepublicIncreased human epididymis protein 4 (HE4) expression was previously observed in lung biopsy specimens of cystic fibrosis (CF). Accordingly,we presumed that serum HE4 concentrations were also elevated in CF, and might be used as a biomarker.In this study, 77 children with CF and 57 adult CF patients were enrolled. In parallel, 94 individuals with non-CF lung diseases, and 117normal controls without pulmonary disorders were analyzed. Serum HE4 was measured by chemiluminescent microparticle immunoassay(Architect?, Abbott). HE4 expression was further investigated via the quantification of HE4 mRNA using RT-qPCR in CF versus non-CF respiratoryepithelium biopsies. The expression of the potential regulator miR-140-5p was analyzed using an UPL-based RT-qPCR assay (Roche). Inaddition, HE4 was measured in the supernatants from unpolarized and polarized cystic fibrosis bronchial epithelial (CFBE) cells expressingWT- or F508del-CFTR.Serum HE4 levels were significantly elevated (P < 0.0001) in both CF children (99.5 [73.1-128.9] pmol/L) and CF adults (115.7 [77.8-148.7]pmol/L) compared to controls (36.3 [31.1-43.4] pmol/L). In contrast, abnormal but lower HE4 concentrations were found in cases of severebronchitis, asthma, pneumonia or bronchiectasis. HE4 concentrations positively correlated with disease severity and C-reactive protein concentrations in CF, while a significant inverse relationship was found between HE4 and the spirometric FEV1 value. Relative HE4 mRNAlevels were significantly augmented (P = 0.011) in the presence of decreased miR-140-5p expression (P = 0.020) in CF versus non-CF airwaybiopsies. Finally, 2-fold higher HE4 concentrations were measured in the supernatants of polarized F508del-CFTR CFBE cells compared toWT cells.In conclusion, serum HE4 positively correlates with the overall severity of CF and the degree of pulmonary dysfunction. Thus, HE4 maybe used as a novel inflammatory biomarker and for the treatment efficacy in this lung disease.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:	Clinical chemistry and laboratory medicine 54 : 10 (2016), p. 184-185. -
További szerzők:	Fila, Libor Clarke, Luka A. Fejes Zsolt (1988-) (molekuláris biológus) Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Kappelmayer János (1960-) (absztraktok) Amaral, Margarida D. Macek Jr., Milan Balogh István (1972-) (molekuláris biológus, genetikus)
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