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001-es BibID:	BIBFORM073020
Első szerző:	Csapó János (agrármérnök)
Cím:	Determination of seleno-amino acids by ion-exchange column chromatography and high-performance liquid chromatography (Preliminary study) / J. Csapó, Cs. Albert
Dátum:	2013
ISSN:	1844-7449 2066-7744
Megjegyzések:	The biological role of selenium was discovered in the sec-ond half of the last century. It was established that selenium was apart of the antioxidant system of the organism, and it protected theorganism from the attack of the harmful free radicals. The researchesmade it clear that the organically bound selenium, like selenomethio-nine (SeMet) and selenocysteine (SeCys), had an especially importantrole. It is very important for experts dealing with both food science andfeeding stuffs to determine the concentration of these two substances infoods and feeding stuffs. We have no information on whether the enan-tiomers of these two seleno-amino acids have ever been examined beforeby anyone else; therefore, we have elaborated new methods and amendedold ones, respectively, for the exact determination of these compounds byion-exchange column chromatography (IEC) and high-performance liquidchromatography (HPLC). In the first step, we carried out the separationof the seleno-amino acids along with protein-components amino acids byion-exchange column chromatography. In the next step, we attemptedthe separation of the oxidized derivatives of these amino acids by IEC,utilizing our experiences gained during the determination of methion-ine (Met) and cysteine (Cys) in oxidized form. Afterwards, we triedto increase the sensibility by applying HPLC after precolumn deriva-tization with OPA/2-mercaptoethanol and OPA/2-mercaptoethane sul-phonic acid, respectively, as the seleno-amino acids are contained in thebiological samples, in a several orders of magnitude lower concentrationas compared to the protein-component amino acids. For the separa-tion of the seleno-amino acid enantiomers, the OPA/TATG derivatiza-tion method was used, which we applied for amino acid enantiomersbeforehand, but derivatization with 1-(9-fluorenyl)-ethyl chloroformate(FLEC) was also attempted.
Tárgyszavak:	Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban selenomethionine selenocysteine determination of seleno-amino acids ion-exchange column chromatography IEC high-performance liquid chromatography HPLC
Megjelenés:	Acta Universitatis Sapientiae, Alimentaria 6 (2013), p. 45-51. -
További szerzők:	Albert Csilla (1976-) (gyógyszerész)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL
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