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001-es BibID:	BIBFORM073121
035-os BibID:	(WoS)000430302400011 (Scopus)85037678277
Első szerző:	Nagy Miklós (vegyész)
Cím:	Novel fluorochromes label tonoplast in living plant cells and reveal changes in vacuolar organization after treatment with protein phosphatase inhibitors / Nagy Miklós, Kéki Sándor, Rácz Dávid, Mathur Jaideep, Vereb György, Garda Tamás, M-Hamvas Márta, Chaumont Francois, Bóka Károly, Böddi Béla, Freytag Csongor, Vasas Gábor, Máthé Csaba
Dátum:	2018
ISSN:	0033-183X
Megjegyzések:	wavelengthsof around 366 nm and bind cysteine-rich proteins with hydrophobic motifs. We show that these compoundspreferentially label tonoplasts in living Arabidopsis and tobacco (Nicotiana tabacum SR1) cells. ACAIN-labeled membranesco-localized with the GFP signal in plants expressing GFP-?-TIP (TIP2;1) (a tonoplast aquaporin) fusion protein.ACAIN preserved the dynamics of vacuolar structures. tip2;1 and triple tip1;1-tip1;2-tip2;1 knockout mutants showedweaker ACAIN signal in tonoplasts. The fluorochrome is also suitable for the labeling and detection of specific(cysteine-rich, hydrophobic) proteins from crude cell protein extracts following SDS-PAGE and TIP mutants showaltered labeling patterns; however, it appears that ACAIN labels a large variety of tonoplast proteins. ACAIN/CACAIN could be used for the detection of altered vacuolar organization induced by the heptapeptide natural toxinmicrocystin-LR (MCY-LR), a potent inhibitor of both type 1 and 2A protein phosphatases and a ROS inducer. Asrevealed both in plants with GFP-TIP2;1 fusions and in wild-type (Columbia) plants labeled with ACAIN/CACAIN,MCY-LR induces the formation of small vesicles, concomitantly with the absence of the large vegetative vacuolescharacteristic for differentiated cells. TEM studies of MCY-LR-treated Arabidopsis cells proved the presence ofmultimembrane vesicles, with characteristics of lytic vacuoles or autophagosomes. Moreover, MCY-LR is a strongerinducer of small vesicle formation than okadaic acid (which inhibits preferentially PP2A) and tautomycin (which inhibitspreferentially PP1). ACAIN and CACAIN emerge as useful novel tools to study plant vacuole biogenesis and programmedcell death.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban ACAIN/CACAIN Arabidopsis Tonoplast Hypocotyl Microcystin-LR Vacuolar organization
Megjelenés:	Protoplasma. - 255 : 3 (2018), p. 829-839. -
További szerzők:	Kéki Sándor (1964-) (polimer kémikus) Rácz Dávid (1987-) (vegyész) Mathur, Jaideep Vereb György (1965-) (biofizikus, orvos) Garda Tamás (1990-) (biológus) Mikóné Hamvas Márta (1963-) (biológus) Chaumont, Francois Bóka Károly (1955-) (Biológus) Böddi Béla Freytag Csongor (1993-) (biológus) Vasas Gábor (1975-) (biológus-vegyész) Máthé Csaba (1966-) (biológus)
Pályázati támogatás:	116465 OTKA K-120638 OTKA GINOP-2.3.2-15-2016-00041 GINOP GINOP-2.3.3-15-2016-00030 GINOP Balassi Institute/Campus Hungary Mobility Support No. B2/2H/7717 Egyéb János Bolyai Research Scholarship MTA Discovery Grant from NSERC, Canada Egyéb
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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