Találati lista | Tudóstér

001-es BibID:	BIBFORM073332
Első szerző:	Lotz Gábor
Cím:	Localization of hepatitis C virus RNA on human erythrocytes by RT in situ PCR technique / G. Lotz, F. Szalay, G. Firneisz, M. Abonyi, G. Lengyel, L. Telegdy, E. Ibrányi, B. Nemes, F. Kury, Z. Schaff, S. Simon
Dátum:	2002
ISSN:	0036-5521
Megjegyzések:	Our previous results showed that hepatitis C virus (HCV) is detectable on erythrocytes by RT in situ PCR. The aims of the present study were to compare the sensitivity of this erythrocyte in situ PCR to routine serum solution phase HCV PCR as well as to obtain more data about the binding and cellular localization of HCV in the erythrocyte. METHODS: 105 previously HCV-infected patients and 20 control individuals were studied using RT in situ PCR on erythrocytes and solution phase RT-PCR from serum samples. Binding of HCV to erythrocytes was studied by in vitro inoculation. RT in situ PCR results were evaluated by fluorescence and confocal laser scanning microscopy. RESULTS: From 105 HCV cases, 78 gave positive, while 5-and all control cases-gave negative results by both PCR techniques. In 21 cases, only the in situ technique provided positive results, while in only I case did the solution phase method provide positive results. During in vitro inoculation, an early HCV-erythrocyte binding was detected followed by virus internalization. CONCLUSIONS: Erythrocyte-in situ PCR was found to show higher sensitivity for the detection of HCV compared to the generally applied serum PCR method. In vitro studies suggested a specific binding of HCV to erythrocyte and showed the virus to be capable of internalization.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Scandinavian Journal Of Gastroenterology 37 : 5 (2002), p. 578-584. -
További szerzők:	Szalay Ferenc Firneisz Gábor Tóth-Abonyi Mihály Lengyel Gabriella Telegdy László Ibrányi Endre Nemes Balázs Áron (1969-) (sebész) Kury F. Schaff Zsuzsa Simon, Susan
Pályázati támogatás:	AKP 98-78 3 Egyéb AKP 98-78 2 Egyéb T 023579 OTKA T 029266 OTKA FKFP 0305/1999 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: