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001-es BibID:	BIBFORM073634
Első szerző:	Vámosi György (biofizikus)
Cím:	Measurement of molecular mobility with fluorescence correlation spectroscopy / György Vámosi, Sándor Damjanovich, János Szöllősi, György Vereb
Dátum:	2009
Megjegyzések:	Fluorescence correlation spectroscopy (FCS) is a ?uctuation method established threedecades ago, whose application to cellular systems became popular in the last decade.Fluctuations of ?uorescence emission are observed from a small, femtoliter to subfemtoliter,usually confocal volume at high time resolution. A time-dependent autocorrelationfunction is generated and evaluated to obtain time constants of photophysicaland photochemical reactions, as well as of molecular diffusion and in the observationvolume. Molecules in various subcellular compartments?including the nucleus, thecytoplasm, and the membrane?can be observed after labeling them with antibodies,ligands, or ?uorescent proteins. The anomaly of diffusion, the local concentration, andthe average ?uorescence per diffusing particle can also be determined, all of which canbe characteristic of molecular interactions. A two-color version of FCS, ?uorescencecross-correlation spectroscopy, can also be applied to observe co-diffusion, i.e., stableassociation of two distinct molecular species in their cellular environment. Curr. Protoc.Cytom. 50:2.15.1-2.15.19.2009 by John Wiley & Sons, Inc.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok könyvfejezet fluorescence correlation spectroscopy FCS fluorescence cross-correlation spectroscopy co-diffusion molecular mobility FCS measurements
Megjelenés:	Current Protocols in Cytometry / ed. J. Paul Robinson. - p. 2.15.1-2.15.19.
További szerzők:	Damjanovich Sándor (1936-2017) (biofizikus) Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (biofizikus, orvos)
Pályázati támogatás:	K62648 OTKA K75752 OTKA K68763 OTKA T48475 OTKA NK61412 OTKA K77600 OTKA K77790 OTKA CK78179 OTKA EU FP6 LSHB-CT-2004503467 Egyéb EU FP6 LSHC-CT-2005-018914 Egyéb EU FP6 MCRTN-CT-035946-2 Egyéb EU FP6 MRTN-CT-2005-019481 Egyéb ETT 066/2006 Egyéb TÁMOP-4.2.2-08/1-2008-0019 TÁMOP
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