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001-es BibID:	BIBFORM074284
Első szerző:	Keserű Judit (molekuláris genetikus)
Cím:	Correlation of plasma mitochondrial DNA copy number and status of ovarian cancer patients / Judit Keserű, Beáta Soltész, János Lukács, Róbert Póka, Bálint Nagy
Dátum:	2018
Megjegyzések:	Introduction: Mitochondria are important organelles in ATP synthesis of cells in aerobic conditions, and are involved in various different cell processes like cell proliferation, and apoptosis. Their copy number variations were connected to many different pathological conditions like development of many types of cancer, e.g. ovarian cancer. That is why we measured the mtDNA copy numbers in the plasma in ovarian cancer patients and healthy controls.Materials and methods: We involved 53 healthy controls (avarage age 57.45?10.61 years) and 24 ovarian cancer patients (average age 61.33?12.72 years) in the study. Blood was drawn into EDTA tubes and plasma was separated by centrifugations. MtDNA copy numbers were determined by Human Mitochondrial DNA (mtDNA) Monitoring Primer Set kit (Takara, Japan) with real-time PCR.Results: The mtDNA copy number was 43.55?13.69 in the healthy control group. We divided the ovarian cancer patients into groups according to their FIGO classification and their cancer stage. The copy numbers were the followings: FIGO I. (early-stage ovarian cancer) 32?8.27; FIGO III. and IV (advanced-stage ovarian cancer) 37.19?13.75 (FIGO III. 33?5.66; FIGO IV. 42?18.87). Student's unpaired t-test was performed. No significant difference was found among the early-stage and advanced-stage patients or control group and advanced stage patients (p=0.3740 and p=0.1084, respectively) but significant difference was found among control group and early-stage patients (p=0.0290) in the plasma cell-free mtDNA copy numbers.Conclusion: We determined the cell-free mtDNA copy numbers in the plasma of ovarian cancer patients and healthy controls and we found significant difference among control group and early-stage patients. We plan to extend the study to more ovarian cancer patients and also we plan exosome encapsulated mtDNA measurements.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt mitochondrial DNA ovarian cancer cell-free mtDNA
Megjelenés:	Biomedical Papers. - 162 : Suppl. 1 (2018), p. S11. -
További szerzők:	Soltész Beáta (1987-) (molekuláris biológus) Lukács János (1975-) (szülész-nőgyógyász, genetikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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