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001-es BibID:	BIBFORM074288
Első szerző:	Szemes, Tomas (biológus)
Cím:	Are phages part of our immunity? / Tomas Szemes, Andrej Balaz, Michal Kajsik, Iveta Gazdaricova, Orsolya Biro, Balint Nagy, Jan Turna, Jaroslav Budis
Dátum:	2018
Megjegyzések:	Introduction: Several non-invasive prenatal tests (NIPT) use total DNA massively parallel sequencing. A portion of sequences are not mapped to human genome reference sequence and is filtered out in the NIPT analysis. Our aim was to determine source of unmapped reads. Preliminary data suggests presence of bacteriophage DNA in human circulation. Materials and methods: We carried out massively parallel sequencing of total DNA from plasma from group of healthy individuals and individuals undergoing antiviral treatment. DNA fragments which mapped to the human reference genome (version hg38) were filtered. We assigned each unmapped fragment a taxonomic label using metagenomic classifier Clark. Taxonomic composition of samples was compared and visualized using Krona graphs. We assembled filtered reads using assembler specialized for assembly of viral genomes called Savage.Results: We identified presence of phage sequences in a small number of reads in studied samples of healthy individuals. In addition, we observed significant drop in phage sequences in individuals undergoing antiviral treatment. In the results we present breakdown of the identified phage data.Conclusions: Preliminary data suggest presence of phage DNA in circulating free nucleic acids. We hypothesize that host immune system tolerates phages to assist in control and stabilization of microenvironment. More rigorous analysis needs to be carried out and supportive experiments are needed to confirm the finding as well as to study our hypotheses.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt DNA phage MPS
Megjelenés:	Biomedical Papers. - 162 : Suppl. 1 (2018), p. S14. -
További szerzők:	Balaz, Andrej Kajsik, Michal Gazdaricova, Iveta Biró Orsolya (molekuláris biológus) Nagy Bálint (1956-) (molekuláris genetikus) Turna, Jan Budis, Jaroslav
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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