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001-es BibID:	BIBFORM074289
Első szerző:	Buglyó Gergely (genetikus)
Cím:	Novel miRNA downregulations in blastemal Wilms' tumor : an FFPE-based study / Gergely Buglyó, Zsófia Magyar, Éva Romicsné Görbe, Rita Bánusz, Monika Csóka, Tamás Micsik, Zsanett Berki, Péter Varga, Zoltán Sápi, Bálint Nagy
Dátum:	2018
Megjegyzések:	According to the SIOP protocol, Wilms' tumor histology is assessed from the surgical sample, after pre-operative chemotherapy. The two most common subtypes are regressive and blastemal. As the presence of blastema is a sign of adverse prognosis, recent efforts have focused on revealing molecular features of blastemal Wilms' tumors that may be responsible for poor chemo-responsiveness. Characteristic miRNA-signatures can be identified from tumor tissue as well as circulating miRNA from serum. In our study, we investigated miRNA expression in 8 patients using FFPE samples from tumor and control tissues. After identifying miRNAs of interest in the first sample by aqRT-PCR Array, 4 chosen miRNAs (miR-184, miR-203a, miR-34c-5p and miR-194-5p) were studied in all samples using individual primers. Results were comparable to previous reports based on fresh frozen tissue, but also showed a deregulation of miR-203a as a novel finding, and an underexpression of miR-184 at a previously unreported magnitude that may be present in a subset of blastemal or other Wilms' tumors. As a conclusion, we may note that further investigation of miRNA expression patterns in Wilms' tumor subtypes may be warranted, and FFPE samples (not older than 7-10 years) are excellent miRNA sources to increase sample count for high-scale studies.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt miRNA Wilms tumor FFPE
Megjelenés:	Biomedical Papers. - 162 : Suppl. 1 (2018), p. S15. -
További szerzők:	Magyar Zsófia Görbe Éva Bánusz Rita Csóka Mónika Micsik Tamás Berki Zsanett Varga Péter (szülész-nőgyógyász) Sápi Zoltán Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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