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001-es BibID:	BIBFORM074292
Első szerző:	Soltész Beáta (molekuláris biológus)
Cím:	Cell-free, long non-coding RNA as novel biomarker in the diagnosis of ovarian cancer / B. Soltesz, J. Lukács, E. Szilágyi, R. Póka, B. Nagy
Dátum:	2018
ISSN:	1018-4813
Megjegyzések:	Background: Ovarian cancer is the fifth leading cause of cancer-associated mortality among women. A reliable, non-invasive diagnostic method may contribute to the early detection of this malignancy. The long non-coding RNAs (lncRNAs) have a significant role in the oncogenesis, metastasis and chemoresistance. The upregulation of Hox transcript antisense intergenic RNA (HOTAIR) was determined in the development of ovarian cancer cells, however, the cell-free HOTAIR expression was not detected from the plasma until now.Materials and methods: Twenty previously untreated ovarian cancer patients (60.60?10.84y; FIGO stages III or IV) and 20 healthy controls (56.70?14.51y) were involved in the study. EDTA blood was drawn; RNA was isolated; cDNA was synthesised and the HOTAIR lncRNA expression were determined by using ExiLERATE LNA? qPCR,for mRNA and long non-coding RNA (Exiqon, USA). ACTB was used as reference gene. Student t-test was applied for the statistical calculations.Results: Higher expression of HOTAIR was detected in the samples of patients with ovarian cancer (1.89?2.39) than in healthy controls (1.39?1.48) but the difference was no significant (p=0.432).Conclusion: We determined the concentration of the cell free HOTAIR lncRNA from the plasma of ovarian cancer patients and healthy controls. There was no significant difference in the expression of the analysed lncRNA; but this is the first study to analyse the cell-free HOTAIR expression among Hungarian ovarian cancer patients. We would like to extend our study on higher number of cases as this lncRNA seem to be novel biomarker for the diagnosis of ovarian cancer.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt ovarian cancer cell-free long-noncoding RNA
Megjelenés:	Biomedical Papers. - 162 : Suppl. 1 (2018), p. S12. -
További szerzők:	Lukács János (1975-) (szülész-nőgyógyász, genetikus) Szilágyi Edina (1993-) (laboratóriumi analitikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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