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001-es BibID:	BIBFORM075134
035-os BibID:	(WoS)000444060600014 (Scopus)85052296237
Első szerző:	Bene László (biofizikus)
Cím:	Dual-Laser Tetra-Polarization FRET (4polFRET) for Site-Selective Control of Homo-FRET in Hetero-FRET Systems on the Cell Surface : the Homo-FRET Gate / Bene L., Bagdány M., Ungvári T., Damjanovich L.
Dátum:	2018
ISSN:	0003-2700
Megjegyzések:	The effects of donor homo-Förster resonance energy transfer (homo-FRET) taking place in hetero-FRET systems is described in the context of hetero-FRET detection via donor and acceptor fluorescence anisotropies in cell surface receptor clusters. Donor homo-FRET can influence both the efficiency of detection as well as the magnitude of the detectable hetero-FRET. A 4-fold polarized FRET detection scheme-tetrapolarization FRET (4polFRET)-is proposed not only for discriminating the effects of homo-FRET from those of hetero-FRET, but also for correlating homo-associations of the donors and acceptors at different donor-acceptor distances, even beyond the critical Förster distance for hetero-FRET ( R0). The method is based on suppressing homo-FRET at the donor side with red-edge excitation. After the anisotropy effects of physical rotation and homo-FRET were separated by site-selective spectroscopy, the magnitude of the effect of homo-FRET on hetero-FRET has been estimated. It has been found significant, offering a new sensitive technique for detecting conformational dynamics via the homo-FRET mediated component of hetero-FRET, the "homo-FRET enhanced hetero-FRET" or "homo-FRET gate". The method is realizable in flow, as well as in image cytometry equipped with polarization detecting facility.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk four-fold fluorescence anisotropy correlations dual-hetero-FRET dual-homo-FRET homo-FRET-sensitized hetero-FRET donor fluorescence anisotropy acceptor fluorescence anisotropy
Megjelenés:	Analytical Chemistry. - 90 : 17 (2018), p. 10159-10170. -
További szerzők:	Bagdány Miklós Ungvári Tamás Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:	TÁMOP-4.2.2.A-11/1/KONV-2012-0045 TÁMOP Sebészet Kutatócsoport
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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