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001-es BibID:	BIBFORM077070
035-os BibID:	(cikkazonosító)e0139659
Első szerző:	Nielsen, Helle H.
Cím:	The Urine Proteome Profile Is Different in Neuromyelitis Optica Compared to Multiple Sclerosis : a Clinical Proteome Study / Helle H. Nielsen, Hans C. Beck, Lars P. Kristensen, Mark Burton, Tunde Csepany, Magdolna Simo, Peter Dioszeghy, Tobias Sejbaek, Manuela Grebing, Niels H. H. Heegaard, Zsolt Illes
Dátum:	2015
ISSN:	1932-6203 1932-6203
Megjegyzések:	OBJECTIVES: Inflammatory demyelinating diseases of the CNS comprise a broad spectrum of diseases like neuromyelitis optica (NMO), NMO spectrum disorders (NMO-SD) and multiple sclerosis (MS). Despite clear classification criteria, differentiation can be difficult. We hypothesized that the urine proteome may differentiate NMO from MS. METHODS: The proteins in urine samples from anti-aquaporin 4 (AQP4) seropositive NMO/NMO-SD patients (n = 32), patients with MS (n = 46) and healthy subjects (HS, n = 31) were examined by quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) after trypsin digestion and iTRAQ labelling. Immunoglobulins (Ig) in the urine were validated by nephelometry in an independent cohort (n = 9-10 pr. groups). RESULTS: The analysis identified a total of 1112 different proteins of which 333 were shared by all 109 subjects. Cluster analysis revealed differences in the urine proteome of NMO/NMO-SD compared to HS and MS. Principal component analysis also suggested that the NMO/NMO-SD proteome profile was useful for classification. Multivariate regression analysis revealed a 3-protein profile for the NMO/NMO-SD versus HS discrimination, a 6-protein profile for NMO/NMO-SD versus MS discrimination and an 11-protein profile for MS versus HS discrimination. All protein panels yielded highly significant ROC curves (AUC in all cases >0.85, p?0.0002). Nephelometry confirmed the presence of increased Ig-light chains in the urine of patients with NMO/NMO-SD. CONCLUSION: The urine proteome profile of patients with NMO/NMO-SD is different from MS and HS. This may reflect differences in the pathogenesis of NMO/NMO-SD versus MS and suggests that urine may be a potential source of biomarkers differentiating NMO/NMO-SD from MS.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plos One. - 10 : 10 (2015), p. 1-15. -
További szerzők:	Beck, Hans C. Kristensen, Lars P. Burton, Mark Csépány Tünde (1956-) (neurológus, pszichiáter) Simó Magdolna Diószeghy Péter (1948-) (ideg- és elmeszakorvos) Sejbaek, Tobias Grebing, Manuela Heegaard, Niels H. H. Illés Zsolt (neurológus, Pécs)
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