Találati lista | Tudóstér

001-es BibID:	BIBFORM078538
035-os BibID:	(WoS)000513525100008 (Scopus)85067838371
Első szerző:	Sikura Katalin Éva (biológus)
Cím:	Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease / Katalin Éva Sikura, László Potor, Tamás Szerafin, Melinda Oros, Péter Nagy, Gábor Méhes, Zoltán Hendrik, Abolfazl Zarjou, Anupam Agarwal, Niké Posta, Roberta Torregrossa, Matthew Whiteman, Ibolya Fürtös, György Balla, József Balla
Dátum:	2020
ISSN:	0007-1188
Megjegyzések:	BACKGROUND AND PURPOSE: Calcification of heart valves is a frequent pathological finding in CKD patients and in elderly. Hydrogen sulfide (H2 S) has been suggested to possess various anti-calcific actions. We aimed to investigate H2 S as a potential therapeutic in valvular calcification and to identify its targets in the pathogenesis. EXPERIMENTAL APPROACH: Potential of H2 S for regulating osteoblastic transdifferentiation of valvular interstitial cells (VIC) isolated from human aortic valves were studied and tested for valvular calcification in apolipoprotein E-deficient mice (ApoE-/- ). KEY RESULTS: In human VIC H2 S treatment employing donors (NaSH, Na2 S, GYY4137, AP67, AP72) inhibited mineralization/osteoblastic transdifferentiation in a dose-responsive manner in response to phosphate. Accumulation of calcium in the extracellular matrix and expression of osteocalcin and alkaline phosphatase was abrogated. Nuclear translocation of the RUNX2 did not occur, and phosphate uptake was lowered. We also found that pyrophosphate generation was increased via up-regulating ENPP2 and ANK1. Lowering endogenous production of H2 S by concomitant silencing of CSE and CBS favored VIC calcification. IHC and Western blot analysis of human specimens revealed higher levels of CSE expression in aorta stenosis valves with calcification (AS) compared to valves of aorta insufficiency (AI). In contrast, tissue H2 S generation was lower in AS valves compared to AI valves. We observed an inhibition of valvular calcification by H2 S in ApoE-/- mouse on high-fat diet. CONCLUSION AND IMPLICATIONS: Our study suggests that the CSE-CBS/H2 S system exhibits an anti-calcification function in heart valves providing a novel therapeutic approach to prevent hardening of valves. This article is protected by copyright. All rights reserved. KEYWORDS: AP67; AP72; GYY4137; H2S; aortic valve; apolipoprotein E knockout mice; calcification; pyrophosphate
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk hydrogen sulphide valvular calcification
Megjelenés:	British Journal of Pharmacology. - 177 : 4 (2020), p. 793-809. -
További szerzők:	Potor László Szerafin Tamás (1960-) (szívsebész, mellkassebész) Oros Melinda (1975-) (molekuláris biológus) Nagy Péter (1976-) (vegyész) Méhes Gábor (1966-) (patológus) Hendrik Zoltán (1986-) (orvos) Zarjou, Abolfazl (1979-) (kutató orvos) Agarwal, Anupam Posta Niké Torregrossa, Roberta Whiteman, Matthew Fürtös Ibolya Balla György (1953-) (csecsemő és gyermekgyógyász, neonatológus) Balla József (1959-) (belgyógyász, nephrológus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00043 GINOP EFOP-3.6.2-16-2017-00006 EFOP OTKA-112333 OTKA 11003 MTA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: